|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||313264 Da|
|Homology||Human, mouse - identical.|
|Other Names||Inositol 1, 5-trisphosphate receptor type 1, IP3 receptor isoform 1, IP-3-R, IP3R 1, InsP3R1, Type 1 inositol 1, 5-trisphosphate receptor, Type 1 InsP3 receptor, Itpr1, Insp3r|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)RIGLLGHPPHMNVNPQQPA, corresponding to amino acid residues 2732-2750 of rat IP3 Receptor-1 (Accession P29994). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Application Details||Western blot analysis (WB): - Rat brain lysates (1:4000) (see Kaya, L. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.3 mg/ml.|
|Buffer After Reconstitution|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1־¼g antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Inositol 1,4,5-triphosphate (IP3) serves as a second messenger for many neurotransmitters, hormones and growth factors.1 The binding of IP3 to its receptor (IP3R), which is a ligand gated Ca2+ channel, located predominantly at the endoplasmic reticulum, results in a rapid release of Ca2+ from intracellular stores.1 To date, three known isoforms of IP3R are known (designated IP3R1, IP3R2, and IP3R3) that can work as homotetramers or hetrotetramers. All three receptors have six transmembrane domains and a pore domain between TM5 and TM6. The N-terminus as well as the C-terminus are facing the cytoplasm. Each IP3R consists of an N-terminal ligand binding domain (LBD) and a C-terminal domain which is linked by a long regulatory domain. The C-terminus is constitutively active, suggesting that the regulatory domain is required in order to maintain the suppression of channel activity.2 IP3R1 is one of the most important channels responsible for Ca2+ release from intracellular stores and was shown to be the predominant isoform expressed in the central nervous system. IP3R1 is a pivotal player in many neuronal functions such as neuronal plasticity, neurite extension and others.3
1. Blondel, O. et al. (1993) J. Biol. Chem. 268, 11356.
2. V'arnai, P. et al. (2005) PNAS. 102, 7859.
3. Cai, W. et al. (2004) J. Biol. Chem. 279, 23691.
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