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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   TRPC4 Antibody   

TRPC4 Antibody

Affinity purified polyclonal antibody

  • WB - TRPC4 Antibody AG1356-025
    Western blot analysis of rat brain membranes:
    1. Anti-TRPC4 antibody (#AG1356), (1:200).
    2. Anti-TRPC4 antibody, preincubated with the control peptide antigen.
  • WB - TRPC4 Antibody AG1356-025
    Western blot analysis of PC3 (lanes 1,3) and LNCaP cell lysates:
    1. Anti-TRPC4 antibody (#AG1356), (1:200).
    2. Anti-TRPC4 antibody, preincubated with the control peptide antigen.
  • WB - TRPC4 Antibody AG1356-025
    Immunoprecipitation of rat brain lysate:
    1. Rat brain lysate.
    2. Lysate immunoprecipitated with Anti-TRPC4 antibody (#AG1356), (6 mg).
    3. Lysate immunoprecipitated with pre-immune rabbit serum.
    The upper arrow indicates the TRPC4 channel while the lower arrow indicates the IgG heavy chain.
    Western blot analysis was performed with Anti-TRPC4 antibody.
  • IHC - TRPC4 Antibody AG1356-025
    Expression of TRPC4 in mouse cerebellum Immunohistochemical staining of mouse cerebellum frozen sections with Anti-TRPC4 antibody (#AG1356). A. TRPC4 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B. Staining with mouse anti-parvalbumin (PV) in the same brain section. C. Confocal merge of TRPC4 and PV demonstrates partial co-localization in the Purkinje and the molecular layers.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9QUQ5
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 111575 Da
Homology Rat, human - 15/16 amino acid residues identical; bovine - 11/15 amino acid residues identical.
Additional Information
Gene ID 22066
Other Names Short transient receptor potential channel 4, TrpC4, Capacitative calcium entry channel Trp4, Receptor-activated cation channel TRP4, Trpc4, Trrp4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4 (Accession number Q9QUQ5).ֲ ֲ Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details Western blot analysis (WB): - Rat INS-1 cell lysate (see Park, S.H. et al. (2013) in Product Citations). - Mouse brain lysate (see Feng, S. et al. (2013) in Product Citations). - Human primary myotubes (see Harisseh, R. et al. (2013) in Product Citations). - Rat distal pulmonary smooth muscle cell lysate (PASMCs), (see Zhang, Y. et al.  (2013) in Product Citations).   Immunocytochemistry (IC): - Human primary myotubes (see Harisseh, R. et al. (2013) in Product Citations). - Rat type I astrocytes (1:25) (see Grimaldi, A. et al. (2003) in Product Citations). - Human U373 MG cells (1:100) (see Barajas, M. et al. (2008) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.4 mg/ml
Buffer After Reconstitution
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4  The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. TRPC1 2. TRPC4 and TRPC5 3. TRPC3, TRPC6, TRPC7 4. TRPC2.2,5  They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC4 can form heterotetramers with TRPC1. TRPC4, TRPC1 and TRPC5 can be activated either by calcium store depletion or by GPCR stimulation pathways and are also assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells. 6


References 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 3. Clapham, D.E. (2003) Nature 426, 517. 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. 6. Liu, X. et al. (2003) J. Biol.Chem. 278, 11337.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1356-025
(40 western blots)
Availability: 2-3 weeks
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