|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||111575 Da|
|Homology||Rat, human - 15/16 amino acid residues identical; bovine - 11/15 amino acid residues identical.|
|Other Names||Short transient receptor potential channel 4, TrpC4, Capacitative calcium entry channel Trp4, Receptor-activated cation channel TRP4, Trpc4, Trrp4|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KEKHAHEEDSSIDYDL, corresponding to amino acid residues 943-958 of mouse TRPC4 (Accession number Q9QUQ5).ֲ ֲ Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat INS-1 cell lysate (see Park, S.H. et al. (2013) in Product Citations). - Mouse brain lysate (see Feng, S. et al. (2013) in Product Citations). - Human primary myotubes (see Harisseh, R. et al. (2013) in Product Citations). - Rat distal pulmonary smooth muscle cell lysate (PASMCs), (see Zhang, Y. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Human primary myotubes (see Harisseh, R. et al. (2013) in Product Citations). - Rat type I astrocytes (1:25) (see Grimaldi, A. et al. (2003) in Product Citations). - Human U373 MG cells (1:100) (see Barajas, M. et al. (2008) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.4 mg/ml|
|Buffer After Reconstitution|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4 The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. TRPC1 2. TRPC4 and TRPC5 3. TRPC3, TRPC6, TRPC7 4. TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues. TRPC4 can form heterotetramers with TRPC1. TRPC4, TRPC1 and TRPC5 can be activated either by calcium store depletion or by GPCR stimulation pathways and are also assumed to form components of store operated channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells. 6
References 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 3. Clapham, D.E. (2003) Nature 426, 517. 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. 6. Liu, X. et al. (2003) J. Biol.Chem. 278, 11337.
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