|Reactivity||Human, Mouse, Rat|
|Calculated MW||242784 Da|
|Homology||Rat, guinea pig - 31/46 amino acid residues identical.|
|Other Names||Voltage-dependent L-type calcium channel subunit alpha-1C, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, Smooth muscle calcium channel blocker receptor, CACB-receptor, Voltage-gated calcium channel subunit alpha Cav12, CACNA1C, CACH2, CACN2, CACNL1A1, CCHL1A1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||GST fusion protein with residues 1-46 of rabbit Cav1.2a, with serine 44 replaced with alanine (Accessionֲ P15381). Intracellular, N-terminus.|
|Peptide Confirmation||Fusion protein confirmed by DNA sequence and by SDS-PAGE.|
|Application Details||Immunohistochemistry (IH): - Human cardiac tissue (see Crossman, D.J. et al. (2011) in Product Citations). Immunocytochemistry (IC): - c-kit+ cells from mouse bone marrow (see Lagostena, L. et al. (2005) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.95 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl PBS.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||3 µg fusion protein per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. Several peptidyl toxins are described that are specific L-type channels blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#C-650), Calciseptine (#C-500) and FS-2 (#F-700). All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptor. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), CaV1.3 channels are found in heart, brain and pancreas and CaV1.2 channels are ubiquitously expressed. There are two splice variants to the Cav1.2 channel designated Cav1.2a and Cav1.2b. The expression of the Cav1.2b variant is restricted to smooth muscle while Cav1.2a specifically expressed in cardiac muscle.
References 1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579. 2. IUPHAR 3. Hu, X.Q. et al. (1998) J. Biol. Chem. 273, 5337. 4. Kreuzberg, U. et al. (2000) Am J. Physiol. 278, H723. 5. Allard, B. et al. (2000) J. Biol. Chem. 275, 25556. 6. Keef, K.D. et al. (2001) Am. J. Physiol. 281, C1743.
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