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>   home   >   Products   >   Primary Antibodies   >   CaV1.2a Antibody   

CaV1.2a Antibody

Affinity purified polyclonal antibody

  • WB - CaV1.2a Antibody AG1357-050
    Western blot analysis of rat heart membranes: 1. Anti-Cav1.2a antibody (#AG1357), (1:200).   
    2. Anti-Cav1.2a antibody, preincubated with the control peptide antigen.
  • IHC - CaV1.2a Antibody AG1357-050
    Expression of Cav1.2a in rat heart Immunohistochemical staining of rat heart with Anti-Cav1.2a antibody (#AG1357). Cav1.2a was visualized with immuno-peroxidase methods and final brown-black diaminobenzidine color product (arrows in A). Cresyl violet is used as the counterstain. When the antibody was pre-incubated with the control peptide antigen, staining was blocked (B).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P15381
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 242784 Da
Homology Rat, guinea pig - 31/46 amino acid residues identical.
Additional Information
Gene ID 100101555
Other Names Voltage-dependent L-type calcium channel subunit alpha-1C, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, Smooth muscle calcium channel blocker receptor, CACB-receptor, Voltage-gated calcium channel subunit alpha Cav12, CACNA1C, CACH2, CACN2, CACNL1A1, CCHL1A1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity GST fusion protein with residues 1-46 of rabbit Cav1.2a, with serine 44 replaced with alanine (Accessionֲ  P15381). Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Fusion protein confirmed by DNA sequence and by SDS-PAGE.
Application Details Immunohistochemistry (IH): - Human cardiac tissue (see Crossman, D.J. et al. (2011) in Product Citations). Immunocytochemistry (IC): - c-kit+ cells from mouse bone marrow (see Lagostena, L. et al. (2005) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.95 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl PBS.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 3 µg fusion protein per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells.   Several peptidyl toxins are described that are specific L-type channels blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#C-650), Calciseptine (#C-500) and FS-2 (#F-700).   All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptor. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), CaV1.3 channels are found in heart, brain and pancreas and CaV1.2 channels are ubiquitously expressed.   There are two splice variants to the Cav1.2 channel designated Cav1.2a and Cav1.2b. The expression  of the Cav1.2b variant is restricted to smooth muscle while Cav1.2a specifically expressed in cardiac muscle.


References 1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579. 2. IUPHAR 3. Hu, X.Q. et al. (1998) J. Biol. Chem. 273, 5337. 4. Kreuzberg, U. et al. (2000) Am J. Physiol. 278, H723. 5. Allard, B. et al. (2000) J. Biol. Chem. 275, 25556. 6. Keef, K.D. et al. (2001) Am. J. Physiol. 281, C1743.

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$ 367.00
$ 475.00
Cat# AG1357-050
Availability: 2-3 weeks
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