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CaVgamma2 Antibody

Affinity purified polyclonal antibody

  • WB - CaVgamma2 Antibody AG1358-050
    Western blot analysis of rat brain membranes:
    1. Anti-Cavγ2 antibody (#AG1358), (1:200).
    2.  Anti-Cavγ2 antibody, preincubated with the control peptide antigen.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O88602
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 35895 Da
Homology Rat, human - identical.
Additional Information
Gene ID 12300
Other Names Voltage-dependent calcium channel gamma-2 subunit, Neuronal voltage-gated calcium channel gamma-2 subunit, Stargazin, Transmembrane AMPAR regulatory protein gamma-2, TARP gamma-2, Cacng2, Stg
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RATDYLQASAITRIPS, corresponding to amino acid residues 213-228 of mouse Cav־³2 (Accession O88602). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.6 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Research Areas
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The γ subunit protein is an auxiliary subunit of the CaV (voltage dependent calcium) channel multimer complex. γ1 was first identified in skeletal muscle as a part of the proteins complex purified as the DHP receptor. Eight γ subunit isoforms were identified in mammalian genoms and they share some sequence as well as structure homology.1 γ subunit contains four transmembrane helixes, with intracellular N and C termini. Usually their association with the pore-forming α1 subunit results in reduced current amplitude. CaVγ2 is also known as stargazin, and interacts and regulates the activity and trafficking of AMPA glutamate receptors.2 


References 1. Arikkath, J. and Campbell, K.P. (2003) Curr. Opin. Neurobiol. 13, 298. 2. Chen, L. et al. (2000) Nature 408, 936.

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$ 475.00
$ 575.00
Cat# AG1358-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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