|Reactivity||Human, Mouse, Rat|
|Calculated MW||243693 Da|
|Homology||Human - 14/15 amino acid residues identical.|
|Other Names||Voltage-dependent T-type calcium channel subunit alpha-1I, CaVT3, Voltage-gated calcium channel subunit alpha Cav33, Cacna1i|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CNGRMPNIAKDVFTK,ֲ ֲ corresponding to amino acid residues 1053-1067 of rat Cav3.3ֲ (Accession number Q9Z0Y8). Intracellular, between domains II and III.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Immunohistochemistry (IH): - Mouse hindbrain (see Moruzzi, A.M. et al. (2009) in Product Citations). Immunocytochemistry (IC): - Human sperm cells (1:50) (see Zhang, D. et al. (2006) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
T-type Ca2+ channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation. T-type channels are also known to participate in the pacemaker activities of heart and neurons including thalamic neurons.1 Three genes encoding T-type Ca2+ channels have been cloned and designated as CaV3.1 (α1G), CaV3.2 (α1H) and CaV3.3 (α1I).1-3 While CaV3.1 (α1G) and CaV3.2 (α1H) are widely expressed in various tissues, CaV3.3 (α1I) is primarily expressed in the central nervous system, where high expression was described in thalamic neurons. The Ca2+ current generated by the CaV3.3 channel displays much slower activation and inactivation compared to the currents produced by CaV3.1 and CaV3.2, suggesting it might play a different role in neuronal excitability.1,4
References 1. Park, J.Y. et al. (2004) J. Biol. Chem. 279, 21707. 2. Chemin, J. et al. (2001) Eur. J. Neurosci. 14, 1678. 3. Monteil, A. et al. (2000) J. Biol. Chem. 275, 16530. 4. Gomora, J.C. et al. (2002) Biophys. J. 83, 229.
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