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CaVbeta3 Antibody

Affinity purified polyclonal antibody

  • WB - CaVbeta3 Antibody AG1360-050
    Western blot analysis of rat brain membranes:
    1.  Anti-Cavβ3 antibody (#AG1360), (1:200).
    2.  Anti-Cavβ3 antibody, preincubated with the control peptide antigen.
  • IHC - CaVbeta3 Antibody AG1360-050
    Expression of Cavβ in rat hippocampus Immunohistochemical staining of rat hippocampus with Anti-Cavβ3 antibody (#AG1360). A. Cavβ3 (red) appears in neurons (arrows). B. Staining of nerve cells with mouse anti-parvalbumin demonstrates the restriction of Cavβ3 to cell bodies. C. Merged image of panels A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P54286
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 53814 Da
Homology Rat, mouse, human, Xenopus - identical; bovine - 14/15 amino acid residues identical.
Additional Information
Gene ID 100009402
Other Names Voltage-dependent L-type calcium channel subunit beta-3, CAB3, Calcium channel voltage-dependent subunit beta 3, CACNB3, CACNLB3
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DRNWQRNRPWPKDSY, corresponding to amino acid residues 463-477 of rabbit Cav־²3 (Accession P54286). Intracellular, C-terminus.Identical for all splice forms.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details   Immunocytochemistry (IC): - A7r5 cell line, derived from the rat thoracic aorta (1:400) (see Uchida, R. et al. (2003) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.3 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
Citations (0)

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The β3 protein is an auxiliary subunit of the CaV (voltage dependent calcium) channel multimer complex. Four β isoforms were identified in mammalian genoms and they share some sequence as well as structure homology.1-3  The structure of CaVβ3 was recentely determined (as well as other CaVβ subunits; for review see reference 3). CaVβ subunits are cytoplasmic proteins, that bind the cytoplasmic face of the pore forming CaV α1 subunits. These interactions have profound effects on channel trafficing to the plasma membrane as well as on current parameters such as voltage dependent kinetics and interactions with modulatory proteins.1,2  CaVβ3 might interact with all the poreforming subunits that have been tested and in vivo is expressed in smooth muscle, trachea, aorta, lung and brain.1  Abgent offers a polyclonal antibody directed against an epitope at the C-terminus of the auxiliary subunit CaVβ3.


References 1. Arikkath, J. and Campbell, K.P. (2003) Curr. Opin. Neurobiol. 13, 298. 2. Dolphin, A.C. (2003) J. Bioenerg. Biomembr. 35, 599. 3. Richards, M.W. et al. (2004) Trends Pharmacol. Sci. 25, 626.

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$ 475.00
$ 575.00
Cat# AG1360-050
(40 western blots)
Availability: 2-3 weeks
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