|Reactivity||Human, Mouse, Rat|
|Calculated MW||53814 Da|
|Homology||Rat, mouse, human, Xenopus - identical; bovine - 14/15 amino acid residues identical.|
|Other Names||Voltage-dependent L-type calcium channel subunit beta-3, CAB3, Calcium channel voltage-dependent subunit beta 3, CACNB3, CACNLB3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DRNWQRNRPWPKDSY, corresponding to amino acid residues 463-477 of rabbit Cav־²3 (Accession P54286). Intracellular, C-terminus.Identical for all splice forms.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Immunocytochemistry (IC): - A7r5 cell line, derived from the rat thoracic aorta (1:400) (see Uchida, R. et al. (2003) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.3 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The β3 protein is an auxiliary subunit of the CaV (voltage dependent calcium) channel multimer complex. Four β isoforms were identified in mammalian genoms and they share some sequence as well as structure homology.1-3 The structure of CaVβ3 was recentely determined (as well as other CaVβ subunits; for review see reference 3). CaVβ subunits are cytoplasmic proteins, that bind the cytoplasmic face of the pore forming CaV α1 subunits. These interactions have profound effects on channel trafficing to the plasma membrane as well as on current parameters such as voltage dependent kinetics and interactions with modulatory proteins.1,2 CaVβ3 might interact with all the poreforming subunits that have been tested and in vivo is expressed in smooth muscle, trachea, aorta, lung and brain.1 Abgent offers a polyclonal antibody directed against an epitope at the C-terminus of the auxiliary subunit CaVβ3.
References 1. Arikkath, J. and Campbell, K.P. (2003) Curr. Opin. Neurobiol. 13, 298. 2. Dolphin, A.C. (2003) J. Bioenerg. Biomembr. 35, 599. 3. Richards, M.W. et al. (2004) Trends Pharmacol. Sci. 25, 626.
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