|Reactivity||Human, Mouse, Rat|
|Calculated MW||252116 Da|
|Homology||Mouse - 14/16 amino acid residues identical; rabbit - 12/16amino acid residues identical; human - 11/16 amino acid residues identical.|
|Other Names||Voltage-dependent R-type calcium channel subunit alpha-1E, BII, Brain calcium channel II, Calcium channel, L type, alpha-1 polypeptide, isoform 6, RBE-II, RBE2, Voltage-gated calcium channel subunit alpha Cav23, Cacna1e, Cach6, Cacnl1a6|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)SASQERSLDEGVSIDG, corresponding to amino acid residues 892-907 of rat CaV2.3 (Accession Q07652).ֲ ֲ Intracellular loop between domains II and III.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.3 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage-dependent Ca2+ channels (Cav channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1 The voltage dependent Ca2+ channels are composed of several subunits; α1, β, α2δ and γ. Cav channels were originally divided into six physiological types: L, N , P, Q, R, and T type.1 The Cav2.3 (formerly named α1E) protein, forms the R type channels. R-type channels are highly sensitive to both SNX-482 (#RTS-500) and NiCl2. Cav2.3 channels were shown to mediate fast excitatory transmission in several synapses including the hippocampus.2 Cav2.3 channels can be modulated by the muscarinic G-protein coupled receptors. In HEK-293 cells, expressing Cav2.3 channels, the R-type channel was shown to be both facilitated and inhibited by m1 and m2 muscarinic receptors. However, inhibition was much weaker than facilitation for the m1 receptor, while for m2 receptors, inhibition was much more pronounced than activation.3 Facilitation and inhibition by the same receptors was probably exerted via different pathways as was indicated by their response to various inhibitors.3
References 1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214. 2. Gasparini, S. et al. (2001) J. Neurosci. 21, 8715. 3. Lanzafame, A.A. et al. (2003) Receptors Channels 9, 241.
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