|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||243482 Da|
|Homology||Cav1 family (α1C, α1D, α1F, α1S) from all known species - identical; Cav2 family (α1A, α1B, α1E) from all known species - 17/19 amino acid residues identical.|
|Other Names||Voltage-dependent L-type calcium channel subunit alpha-1C, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, Rat brain class C, RBC, Voltage-gated calcium channel subunit alpha Cav12, Cacna1c, Cach2, Cacn2, Cacnl1a1, Cchl1a1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DNFDYLTRDWSILGPHHLD, corresponding to amino acid residues 1506-1524 of rat Cav1.2 (Accession P22002). Intracellular, C-terminus region.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Immunoprecipitation (IP): - Rat basilar artery lysates (see Murphy, K. et al. (2003) in Product Citations) Immunohistochemistry (IH): - Crab cardiac ganglion (1:100) (see Ransdell, J.L. et al. (2013) in Product Citations). - Human placenta (1:300) (see Bernucci, L. et al. (2006) in Prodcust Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.3 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl deionized water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Ca2+ ions regulate many cellular processes such as secretion, proliferation, and apoptosis and they are also cofactors of many proteins.1,2 In neurons, Ca2+ entry through voltage-dependent Ca2+ (Cav) channels triggers neurotransmitter release. Fast synaptic transmission in the mammalian central nervous system (CNS) is mediated by several high voltage-dependent Ca2+ channels.3 Five types of Ca2+ channels are expressed in the CNS: the L-type (Cav1), N-type (Cav2.2), P/Q-type (Cav2.1), R-type (Cav2.3), and the T-type (Cav3).4 Each Cav channel is a multimeric protein composed of a pore forming α1 subunit and the auxiliary β (Cavβ), α2δ and γ subunits. There are four known Cavβ subunits, in addition to four α2δ subunits and eight γ subunits.4
References 1. Bonilla, M. and Cunningham, K.M. (2002) Sci. STKE 127, PE17. 2. Wu, X. et al. (2004) J. Biol. Chem. 279, 43392. 3. Iwasaki, S. et al. (2000) J. Neurosci. 20, 59. 4. Mark, M.D. and Herlitze, S. (2000) Eur. J. Biochem. 267, 5830.
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