|Application ||WB, IHC, FC, IP, ICC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||243482 Da|
|Homology||Mouse - identical; guinea pig -17/18 amino acid residues identical; human, rabbit - 16/18 amino acid residues identical.|
|Other Names||Voltage-dependent L-type calcium channel subunit alpha-1C, Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle, Rat brain class C, RBC, Voltage-gated calcium channel subunit alpha Cav12, Cacna1c, Cach2, Cacn2, Cacnl1a1, Cchl1a1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)TTKINMDDLQPSENEDKS, corresponding to amino acid residues 848-865 of rat Cav1.2 (Accession P22002). Intracellular loop between domains II and III.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat myocytes (see Li, J. et al. (2013) in Product Citations). - Rat primary submucosa cells (see Rehn, M. et al. (2013) in Product Citations). - Mouse synaptosome (1:400) (see Saggu, S. et al. (2013) in Product Citations). - Mouse mesenteric and pulmonary arteries (1:200) (see Ko, E.A. et al. (2013) in Product Citations). - HEK-293 cells expressing CaV1.2 (see Boczek, N.J. et al. (2013) in Product Citations). - Human cardiac tissue (see Crossman, D.J. et al. (2011) in Product Citations). Immunoprecipitation (IP): - CaV1.2 transfected HEK-293 cells and mouse heart lysate (see Rougier, J.S. et al. (2011) in Product Citations). Immunohistochemistry (IH): - Rat retinal sections (1:1000) (see Sargoy, A. et al. (2013) in Product Citations). - Rat intracardiac ganglia and stellate ganglia neurons (see Tu, H. et al. (2014) in Product Citations). - Rat retinal sections (1:250-1:500) (see Liu, X. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Rat primary submucosa cells (1:50) (see Rehn, M. et al. (2013) in Product Citations). - Rat insulinoma cells (RIN) (see Parkash, J. (2011) in Product Citations). - Mouse chromaffin cells (1:200). (see Perez-Alvarez, A. et al. (2011) in Product Citations). Indirect flow cytometry (IFC): - Rat mast cells (RBL-2H3 cells, 1μg/5x105 cells). (see Yoshimaru, T. et al. (2009) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl deionized water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptor. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channels blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#C-650), Calciseptine (#C-500) and FS-2 (#F-700).
1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
3. Hu, X.Q. et al. (1998) J. Biol. Chem. 273, 5337.
4. Kreuzberg, U. et al. (2000) Am J. Physiol. 278, H723.
5. Allard, B. et al. (2000) J. Biol. Chem. 275. 25556.
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