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B1 Bradykinin Receptor Antibody

Affinity purified rabbit polyclonal antibody

  • WB - B1 Bradykinin Receptor Antibody AG1366-050
    Western blot analysis of rat brain (1,3) and rat heart (2,4) lysates: 1. Anti-B1 Bradykinin Receptor antibody (#AG1366), (1:200). 2. Anti-B1 Bradykinin Receptor antibody, preincubated with the control peptide antigen.
  • IHC - B1 Bradykinin Receptor Antibody AG1366-050
    Expression of B1 Bradykinin Receptor in rat CNS Immunohistochemical staining of frozen rat brain section (A&B) and cerebellum (C) using Anti-B1 Bradykinin receptor antibody (#AG1366), (1:50). B1 Bradykinin receptor is expressed in neuronal cells of the amygdala (A) and hippocampus (CA1) (B) as well as in the axons of Purkinje cells in the cerebellum (arrowheads on C). Hoechst 33342 is used as the counterstain.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P97583
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 38378 Da
Homology Mouse - 13/15 amino acid residues identical.
Additional Information
Gene ID 81509
Other Names B1 bradykinin receptor, B1R, BK-1 receptor, Kinin B1 receptor, KB1, Bdkrb1, B1bkr, Bkr
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KEAS RTR*SG GPKGS K, corresponding to amino acid residues 243-257 of rat BKRB1 with replacement of cysteine 250 (C250) with serine (*S) (Accession P97583). 3rd intracellular loop.Might not recognize the human epitope.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Immunohistochemistry (IH): - Rat lung sections (see Duehrkop, C. et al. (2013) in Product Citations). - Mouse spinal cord. Also tested in B1R-/- mice (1:200), (see Dutra, R.C. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
Citations (0)

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Kinins are small peptides rapidly produced following tissue injury that serve as important modulators of inflammation and pain. In the periphery, the actions of kinins include vasodilatation, increased vascular permeability, stimulation of immune cells, and induction of pain. Kinins in the central nervous system (CNS) appear to initiate a similar cascade of events leading to neural tissue damage as well as long lasting disturbances affecting blood-brain barrier function.1 Kinins such as Bradykinin (BK), Lys-BK, desArg9-BK, and Lys-desArg9-BK exert their action via two distinct receptors: the B1 Bradykinin receptor (BKRB1) and the B2 Bradykinin receptor (BKRB2). The desArg9-BK and Lys-desArg9-BK peptides activate BKRB1 while BK and Lys-BK operate by activating BKRB2.2 Both BKRB1 and BKRB2 are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus. Expression of BKRB1is inducible upon various types of tissue injury and by inflammatory mediators such as bacterial lipopolysaccharide (LPS) and cytokines. BKRB1 was long considered not to be expressed in healthy tissues. However, recent work has demonstrated a low level of expression of BKRB1 in the CNS of rodent and primates. In contrast, BKRB2 is constitutively expressed on various cell types.3 BKRB1 represents a potential therapeutic target for treatment of inflammatory disorders and cardiovascular diseases.


1. Walker, K. et al. (1995) Neurochem. Int. 26, 1.
2. Böckmann, S. and Paegelow, I. (2000) J. Leukoc. Biol. 68, 587.
3. Hess, J.F. et al. (2004) J. Pharmacol. Exp. Ther. 310, 488.

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$ 475.00
$ 575.00
Cat# AG1366-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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