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Bombesin Receptor 1 Antibody

Affinity purified rabbit polyclonal antibody

     
  • WB - Bombesin Receptor 1 Antibody AG1367-050
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: 1, 2. Anti-Bombesin Receptor 1 antibody (#AG1367), (1:1000). 3, 4. Anti-Bombesin Receptor 1 antibody, preincubated with the control peptide antigen.
  • WB - Bombesin Receptor 1 Antibody AG1367-050
    Western blot analysis of human lung carcinoma NCI-H526 (lanes 1 and 3) and human prostate carcinoma PC-3 (lanes 2 and 4) cell line lysates: 1, 2. Anti-Bombesin Receptor 1 antibody (#AG1367), (1:1000). 3, 4. Anti-Bombesin Receptor 1 antibody, preincubated with the control peptide antigen.
  • IHC - Bombesin Receptor 1 Antibody AG1367-050
    Expression of Bombesin Receptor 1 in rat hypothalamus Immunohistochemical staining of frozen rat hypothalamus sections using Anti-Bombesin Receptor 1 antibody (#AG1367), (1:200). A. Staining (red) appears in the neuropil near the ventricle (asterisk) and in neurons (triangles). Calbindin D28k staining (green) appears in neurons. Merge of (A) and (B) shows co-expression of Bombesin Receptor 1 and Calbindin in a few neurons (vertical arrow). DAPI is used as the counterstain.
  • ICC - Bombesin Receptor 1 Antibody AG1367-050
    Expression of Bombesin Receptor 1 in a human breast cancer cell line Immunocytochemical staining of paraformaldehyde-fixed and permeabilized mammary gland adenocarcinoma MDA-MB-231. A. Cells were stained with Anti-Bombesin Receptor 1 antibody (#AG1367), (1:1000), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). Hoechst 33342 (blue) is used to visulaize the nuclei. B. Live view of the same field as in (A).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC
Primary Accession P28336
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 43435 Da
Homology Mouse, dog - identical; rat - 15/16 amino acid residues identical.
Additional Information
Gene ID 4829
Other Names Neuromedin-B receptor, NMB-R, Epididymis tissue protein Li 185a, Neuromedin-B-preferring bombesin receptor, NMBR
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KSAHNLPGEYNEHTKK, corresponding to amino acid residues 241-256 of human BB1 (Accession P28336). 3rd intracellular loop.
Dilution WB~~1:200-1:2000
IHC~~1:200
ICC~~1:1000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.6 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Background

Bombesin receptor 1 (BB1) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was initially isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP) and neuromedin B (NMB), 27 and 10 amino acid homologues, respectively.1 BB1 is the preferred receptor for NMB (therefore it is also known as NMB receptor) while BB2 is the preferred receptor for GRP.2 BB3 was the third member of the bombesin family of receptors to be cloned based upon its sequence similarity to BB1 and BB2.1 The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors, as are its affinities for NMB and GRP. This suggests that the endogenous mammalian ligand for the BB3 receptor remains to be identified. All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB1 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.1 BB1 is expressed in the brain where it likely mediates the inhibition of food intake and regulates the stress and fear response. BB1 and its ligand NMB are expressed in the pituitary where they participate in the regulation of thyroid-stimulating hormone (TSH) secretion in both autocrine and paracrine fashions.3 In the periphery, BB1 is expressed in the gastrointestinal and urogenital smooth muscle but its physiological function in these tissues is unclear. Finally, BB1 is over-expressed in several human tumors especially colon and non-small lung cancer where it acts as a growth factor receptor inducing tumor growth. BB1 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4 Abgent is pleased to offer a highly specific antibody directed against a well conserved epitope located in the 3rd intracellular loop of the human BB1 receptor. Anti-Bombesin Receptor 1 antibody (#AG1367) can be used in western blot and immunocytochemical applications, and will recognize BB1 from human, rat, mouse, and dog samples.

References

1. Ohki-Hamazaki, H. et al. (2005) Int. J. Dev. Biol. 49, 293.
2. Wada E. et al. (1991) Neuron 6, 421.
3. Oliveira, K.J. et al. (2006) J Mol Endocrinol 36, 73.
4. Reubi, J.C. (2003) Endocr. Rev. 24, 389.

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$ 475.00
$ 575.00
Cat# AG1367-050
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