|Application ||WB, IHC, FC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||44411 Da|
|Homology||Rat, mouse - 13/16 amino acids residues identical.|
|Other Names||Bombesin receptor subtype-3, BRS-3, BRS3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)SNVYTFRDPNKNMTFE, corresponding to amino acid residues 186-201 of human BB3 (Accession P32247). 2nd extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Bombesin receptor 3 (BB3) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was first isolated from frog skin and it was later established that mammalians express endogenous bombesin like peptides such as gastrin-releasing peptide (GRP) a 27 amino acid homologue and neuromedin B (NMB) a 10 amino acid homologue.1 BB1 is the preferred receptor for NMB while BB2 is the preferred receptor for GRP. BB3 was the third member of the bombesin receptors family to be cloned based in its sequence similarity to BB1 and BB2.2 The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors as is its affinity for NMB and GRP. This suggests that the mammalian endogenous ligand for the BB3 receptor remains to be identified. All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB3 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization and cell growth.3 The physiological function of the BB3 receptor is not clear. However, studies with BB3 receptor deficient mice have shown that the mice develop mild obesity associated with hypertension and reduced metabolic rate. 4, 5 Finally, BB3 receptor expression has been identified in several human tumors most notably lung. BB3 is therefore considered a potential target for the development of both diagnostics and anti-cancer therapies.6 Abgent is pleased to present a highly specific antibody directed against a well conserved epitope of the human BB3 receptor. Anti-Bombesin Receptor 3 (extracellular) antibody (#AG1368) can be used in western blot analysis, as well as immunohistochemical and flow cytometry applications, and will recognize BB3 from rat and mouse samples.
1. Ohki-Hamazaki, H. et al. (2005) Int. J. Dev. Biol. 49, 293.
2. Fathi, Z. et al. (1993) J. Biol. Chem. 268, 5979.
3. Ryan, R.R. et al. (1998) J Pharmacol Exp Ther. 287, 366.
4. Ohki-Hamazaki, H. et al. (1997) Nature 390, 165.
5. Nakamichi, Y. et al. (2004) Biochem Biophys Res Commun. 318, 698.
6. Reubi, J.C. (2003) Endocr. Rev. 24, 389.
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