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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   GPCR Antibodies   >   Bombesin Receptor 2 (extracellular) Antibody   

Bombesin Receptor 2 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Bombesin Receptor 2 (extracellular) Antibody AG1369-050
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates: 1, 2. Anti-Bombesin Receptor 2 (extracellular) antibody (#AG1369), (1:500).
    3, 4. Anti-Bombesin Receptor 2 (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - Bombesin Receptor 2 (extracellular) Antibody AG1369-050
    Western blot analysis of human DU-145 (lanes 1 and 3) and PC-3 (lanes 2 and 4) prostate carcinoma cell lines: 1, 2. Anti-Bombesin Receptor 2 (extracellular) antibody (#AG1369), (1:200).
    3, 4. Anti-Bombesin Receptor 2 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Bombesin Receptor 2 (extracellular) Antibody AG1369-050
    Expression of Bombesin Receptor 2 in human colon Immunohistochemical staining of paraffin-embedded human colon using Anti-Bombesin Receptor 2 (extracellular) antibody (#AG1369), (1:50). (A and B) Human colon showing malignant growth. Staining is specific for epithelium-derived malignant cells. (C and D) Normal colon, staining is specific for absorptive epithelial cells in the crypts of Lieberkuhn. Histofine (pink) is used for the color reaction. Hematoxilin is used as the counterstain.
  • ICC - Bombesin Receptor 2 (extracellular) Antibody AG1369-050
    Expression of Bombesin Receptor 2 in human HT-29 cells Immunocytochemical staining of live intact human HT-29 (colorectal adenocarcinoma) cells. Cells were stained with Anti-Bombesin Receptor 2 (extracellular) antibody (#AG1369) (1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody, showing surface expression of the BB2 receptor.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30550
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 43199 Da
Homology Rat, mouse, dog - identical.
Additional Information
Gene ID 2925
Other Names Gastrin-releasing peptide receptor, GRP-R, GRP-preferring bombesin receptor, GRPR
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RSYHYSEVDTSMLH, corresponding to aminoֲ acidֲ residues 287-300 of human BB2 (Accession P30550). 3rd extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Bombesin receptor 2 (BB2) is a member of a family of receptors that binds the 14 amino acid peptide, bombesin. Bombesin was first isolated from frog skin and it was later established that mammals express endogenous bombesin-like peptides such as gastrin-releasing peptide (GRP; a 27 amino acid homologue) and neuromedin B (NMB; a 10 amino acid homologue).   BB2 is the preferred receptor for GRP and hence it is also referred to as GRP receptor or GRP-R.1 BB1 is the preferred receptor for NMB and therefore it is also known as NMB-R. A third member (called BB3) of the bombesin receptor family has been recognized but the endogenous mammalian ligand for this receptor remains to be identified.   All three bombesin receptors are members of the seven-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB2 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization, and cell growth.2   GRP (through its binding to BB2) plays an essential role in the release of hormones in the intestinal tract, the regulation of smooth muscle contraction, and the secretion of pancreatic enzymes.2 In the brain, BB2 has been implicated in regulating feeding behavior, thermoregulation, and memory formation.3   Finally, BB2 is overexpressed in several human tumors including breast, prostate, and lung, where it acts as a growth factor receptor inducing tumor growth. BB2 is considered a potential target for the development of both diagnostics and anti-cancer therapies.4   Abgent is pleased to offer a highly specific antibody directed against an epitope located in the 3rd extracellular loop of the human BB2 receptor. Anti-Bombesin Receptor 2 (extracellular) antibody (#AG1369) can be used in western blot analysis, as well as immunocytochemical and immunohistochemical applications, and will recognize BB2 from human, rat, mouse, and dog samples.


References 1. Battey, J.F. et al. (1991) Proc Natl Acad Sci USA 88, 395. 2. Kroog, G.S. et al.(1995) Med. Res. Rev. 15, 389. 3. Ohki-Hamazaki, H. et al. (2005) Int. J. Dev. Biol. 49, 293. 4. Reubi, J.C. (2003) Endocr. Rev. 24, 389.

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$ 367.00
$ 475.00
Cat# AG1369-050
Availability: 2-3 weeks
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