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C5a Anaphylatoxin Receptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • FC - C5a Anaphylatoxin Receptor (extracellular) Antibody AG1372-050
    Flow cytometric analysis of human peripheral blood granulocytes cells using Anti-C5a Anaphylatoxin Receptor (extracellular) (green, Cat#AG1372) compared to an isotype control of rabbit IgG (blue). AG1372 was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • WB - C5a Anaphylatoxin Receptor (extracellular) Antibody AG1372-050
    Western blot analysis of K562 (lanes 1 and 4), MEG-01 (lanes 2 and 5) and THP-1 (lanes 3 and 6) cell lysates: 1-3. Anti-C5a Anaphylatoxin Receptor (extracellular) antibody (#AG1372), (1:200). 4-6. Anti-C5a Anaphylatoxin Receptor (extracellular) antibody, preincubated with the control peptide antigen.
  • FC - C5a Anaphylatoxin Receptor (extracellular) Antibody AG1372-050
    Indirect flow cytometry analysis of HL-60 intact living cells:
    Black: Unstained cells.
    Green: Cells + Anti-C5a Anaphylatoxin Receptor (extracellular) antibody (#AG1372), (10 µg/1x106 cells).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21730
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 39336 Da
Homology Human only. Not recommended for use with rat and mouse samples.
Additional Information
Gene ID 728
Other Names C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88, C5AR1, C5AR, C5R1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DKDTLDLNTPVDK, corresponding to amino acid residues 16-28 of human C5aR (Accession P21730 ). Extracellular, N-terminus.
Dilution FC~~1:100
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 1 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
Citations (0)

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The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1. C5a is one of the most potent inflammatory peptides generated by complement activation. This peptide has many functions such as acting as a chemoattractant for neutrophils2,3. C5a acts as a vasodilator3,4 and activates the coagulation pathway3,5 just to name a few. C5a exerts its numerous actions by binding to C5aR and C5L2. C5aR belongs to the superfamily of G-protein coupled receptors (GPCRs). Like all members, C5aR has seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. C5aR can form homodimers6-8 or heteromer with CCR5, chemokine receptor, for example8,9. Activation of C5aR by C5a binding causes Ca2+ mobilization10. C5aR expression is detected in neutrophils, eisinophils, basophils and monocytes3,11,12. The receptor is also expressed in lung and liver13,14. Blocking C5aR by peptides or organic small molecules is in the pipeline for treating chronic inflammatory disorders/diseases like arthritis, inflammatory bowel disease and lung/liver injuries8. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human C5aR. Anti-C5a Anaphylatoxin Receptor (extracellular) antibody (#AG1372) can be used in western blot and indirect flow cytometry applications. It has been designed to recognize C5aR from human samples.


1. Haas, P.J. and van Srijp, J. (2007) Immun. Res. 37, 161.
2. Marder, S.R. et al. (1985) J. Immunol. 134, 3325.
3. Guo, R.F. and Ward, P.A. (2005) Annu. Rev. Immunol. 23, 821.
4. Schumacher, W.A. et al. (1991) Agents Actions 34, 345.
5. Laudes, I.J. et al. (2002) Am. J. Pathol. 160, 1867.
6. Geva, A. et al. (2000) J. Biol. Chem. 275, 35393.
7. Klco, J.M. et al. (2003) J. Biol. Chem. 278, 35345.
8. Monk, P.N. et al. (2007) Br. J. Pharmacol. 152, 429.
9. Huttenrauch, F. et al. (2005) J. Biol. Chem. 280, 37503.
10. Ward, P.A. (2009) J. Mol. Med. 87, 375.
11. Gerard, N.P. et al. (1989) J. Biol. Chem. 264, 1760.
12. Werfel, T. et al. (1992) Blood 79, 152.

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$ 475.00
$ 575.00
Cat# AG1372-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
Seasonal Special on Bulk Order
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