|Application ||FC, WB|
|Calculated MW||53864 Da|
|Homology||Recommended to use with human samples only.|
|Other Names||C3a anaphylatoxin chemotactic receptor, C3AR, C3a-R, C3AR1, AZ3B, C3R1, HNFAG09|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EDHETSPLDNSD, corresponding to amino acid residues 276-287 of human C3a anaphylatoxin receptor (Accession Q16581 ). 2nd extracellular loop.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1. Three different pathways are known to activate the complement. The C3 complement plays a key role in all three pathways leading to the activation of the complement. Produced in the liver, C3 is the most abundant complement protein in the serum. It is composed of two polypeptides, an α and β chain. Cleavage of C3 leads to the production of C3b and C3a1. C3a binds to C3a receptor, a G-protein coupled receptor which couples to Gi upon activation. Like all other members of the superfamily, C3a receptor has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail1. The receptor has two glycosylation sites, an unusually large extracellular loop with a sulfated tyrosine residue important for ligand recognition and binding2,3. C3a receptor is expressed in cells of myeloid origin like neutrophils, macrophages and dendritic cells to name a few2. It is also detected in astrocytes from inflamed brain4, endothelial cells5 and activated human T cells6. mRNA of C3a receptor is detected in lung, liver, kidney, brain heart, muscle and testis2,7. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of human C3a receptor. Anti-C3a Anaphylatoxin Receptor (extracellular) antibody (#AG1373) can be used in western blot and indirect flow cytometry applications. It has been designed to recognize C3a receptor from human samples.
1. Haas, P.J. and van Srijp, J. (2007) Immun. Res. 37, 161.
2. Klos, A. et al. (2009) Molec. Immun. 46, 2753.
3. Gao, J. et al. (2003) J. Biol. Chem. 278, 37902.
4. Gasque, P. et al. (1998) J. Immunol. 160, 3543.
5. Monsinjon, T. et al. (2003) FASEB J. 17, 1003.
6. Werfel, T. et al. (1997) Arch. Dermatol. Res. 289, 83.
7. Hsu, M.H. et al. (1997) Immunogenetics 47, 64.
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