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alpha2C-Adrenoceptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

     
  • WB - alpha2C-Adrenoceptor (extracellular) Antibody AG1374-050
    Western blot analysis of rat lung (lanes 1 and 4), kidney (lanes 2 and 5) and brain (lanes 3 and 6) membranes: 1-3. Anti-α2C-Adrenoceptor (extracellular) antibody (#AG1374), (1:200). 4-6. Anti-α2C-Adrenoceptor (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - alpha2C-Adrenoceptor (extracellular) Antibody AG1374-050
    Expression of α2C-Adrenoceptor in rat kidney Immunohistochemical staining of rat kidney paraffin embedded sections using Anti-α2C-Adrenoceptor (extracellular) antibody (#AG1374), (1:100). α2C-Adrenoceptor is expressed in both proximal (PT) and distal (DT) renal tubules and in the collecting ducts (CD). Hematoxilin is used as the counterstain.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P22086
Reactivity Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 49864 Da
Homology Rat, mouse - 15/16 amino acid residues identical; human - 14/16 amino acid residues identical.
Additional Information
Gene ID 24175
Other Names Alpha-2C adrenergic receptor, Alpha-2 adrenergic receptor subtype C4, Alpha-2C adrenoreceptor, Alpha-2C adrenoceptor, Alpha-2CAR, Adra2c
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RRPDGAAYPQ*SGLNDE, corresponding to amino acid residues 192-207 of rat ־±2C-Adrenoceptor  with replacement of cysteine 202 (C202) with serine (*S) (Accession P22086). 2nd extracellular loop.
Dilution WB~~1:200-1:2000
IHC~~1:100
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
Citations (0)

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Background

Adrenoceptors (also called Adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. The Adrenoceptors are members of the G-Protein Coupled Receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular N-terminus, and a cytoplasmic C- terminus.The Adrenoceptors are divided into three types: α1, α2 and β-Adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. The Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2. The α2C-Adrenoceptor subtype was identified by binding studies in the opossum kidney and in cell lines from this organ (OK cells)3,4. Surprisingly, the α2C-Adrenoceptor subtype seems not to play a major role in cardiovascular regulation or the other classical effects of α2-Adrenergic receptors5. Some researchers have suggested that the α2C-Adrenoceptor subtype may play a role in modulating motor behaviour and perhaps in memory processes6,7. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat α2C-Adrenoceptor. Anti-α2C-Adrenoceptor (extracellular) antibody (#AG1374) can be used for western blot and immunohistochemistry applications. It has been designed to recognize α2C-Adrenoceptor from rat, mouse and human samples.

References

1. IUPHAR RECEPTOR DATABASE | ADRENOCEPTORS.
2. Piascik, M.T. and Perez, D. M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
3. Murphy, T.J. and Bylund, D.B. (1988) J. Pharmacol. Exp. Ther. 244, 571.
4. Blaxall, H.S. et al. (1991) J. Pharmacol. Exp. Ther. 2559, 323.
5. Bjorklind M. et al. (1999) Neuroscience 88, 1187.
6. Tanila H. et al. (1999) Eur. J. Neurosci. 11, 599.

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$ 475.00
$ 575.00
Cat# AG1374-050
Size:
Quantity:
(40 western blots)
Availability: 2-3 weeks
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