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alpha2A-Adrenoceptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

     
  • WB - alpha2A-Adrenoceptor (extracellular) Antibody AG1376-025
    Western blot analysis of lysate from human brain tissue lysate, using Adra2a Antibody(Cat. #AG1376). AG1376 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - alpha2A-Adrenoceptor (extracellular) Antibody AG1376-025
    Western blot analysis of lysate from rat brain tissue lysate, using Adra2a Antibody(Cat. #AG1376). AG1376 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • WB - alpha2A-Adrenoceptor (extracellular) Antibody AG1376-025
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: 1, 2. Anti-α2A-Adrenoceptor (extracellular) antibody (#AG1376), (1:200). 3, 4. Anti-α2A-Adrenoceptor (extracellular) antibody, preincubated with the control peptide antigen.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P22909
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 48940 Da
Homology Mouse - identical; human - 13/14 amino acid residues identical.
Additional Information
Gene ID 25083
Other Names Alpha-2A adrenergic receptor, Alpha-2A adrenoreceptor, Alpha-2A adrenoceptor, Alpha-2AAR, Alpha-2D adrenergic receptor, CA2-47, Adra2a
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)DAGNSSWNGTEAPG, corresponding to amino acid residues 7-20 of  rat ־±2A-Adrenoceptor (Accession P22909). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.85 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Background

Adrenoceptors (also called Adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. The Adrenoceptors are members of the G-Protein Coupled Receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular N-terminus, and a cytoplasmic C- terminus.The Adrenoceptors are divided into three types: α1, α2 and β-Adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. The Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2. In general, the pharmacological properties of each subtype are quite homogenous across different species. However, this is not the case for the α2A subtype which was first isolated from human platelets3. This subtype shows different pharmacological properties from that of mouse and rat. For this reason, until molecular techniques significantly advanced, it was believed that human α2A and that of mouse and rat (termed α2D) were two different subtypes. Today, it is accepted that these two subtypes are in fact one gene product and is generally termed α2A. One of the main differences between this subtype from the different organisms is its affinity for yohimbine and rauwolscine; rat and mouse α2A displays less affinity compared to human3,4. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat α2A-Adrenoceptor. The Anti-α2A-Adrenoceptor (extracellular) antibody (#AG1376) can be used for western blot analysis. It has been designed to recognize α2A-Adrenoceptor from mouse, rat and human samples.

References

1. IUPHAR RECEPTOR DATABASE | ADRENOCEPTORS
2. Piascik, M. T .and Perez, D. M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
3. Civantos Calzada, B. and De Artinano, A.A. (2001) Pharmacol. Res. 44, 195.
4. Bylund, D.B. (2005) Br. J. Pharmacol. 144, 159.

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Cat# AG1376-025
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