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alpha1D-Adrenoceptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - alpha1D-Adrenoceptor (extracellular) Antibody AG1377-050
    Western blot analysis of rat brain stem (lanes 1 and 3), mouse brain (lanes 2 and 4), SH-SY5Y (lanes 5 and 7) and Jurkat (lanes 6 and 8) lysates: 1, 2, 5, 6. Anti-α1D-Adrenoceptor (extracellular) antibody (#AG1377), (1:200). 3, 4, 7, 8. Anti-α1D-Adrenoceptor (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - alpha1D-Adrenoceptor (extracellular) Antibody AG1377-050
    Expression of α1D-Adrenoceptor in rat heart Immunohistochemical staining of rat heart paraffin embedded sections using Anti-α1D-Adrenoceptor (extracellular) antibody (#AG1377), (1:100). α1D-Adrenoceptor is expressed in cardiomyocytes of the myocardium and in the smooth muscle of the blood vessels (arrows). Hematoxilin is used as the counterstain.
  • IHC - alpha1D-Adrenoceptor (extracellular) Antibody AG1377-050
    Expression of α1D-Adrenoceptor in rat neocortex Immunohistochemical staining of α1D-Adrenoceptor in rat neocortex using Anti-α1D-Adrenoceptor (extracellular) antibody (#AG1377). A. Most intense staining of α1D-Adrenoceptor (green) appears in apical dendrites (thin horizontal arrows) but also in the soma (thick horizontal arrow). Few cortical interneurons express α1D-Adrenoceptor. B. The same section was also stained for parvalbumin and one cell (marked with a vertical arrow) also expresses α1D-Adrenoceptor.
  • FC - alpha1D-Adrenoceptor (extracellular) Antibody AG1377-050
    Indirect flow cytometry analysis of intact live Jurkat cells:
    Black: Unstained cells.
    Green: Cells + Anti-α1D-Adrenoceptor (extracellular) antibody (#AG1377), (5-10 µg antibody/0.5x106 cells).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P23944
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 59354 Da
Homology Rat, human, mouse - 14/15 amino acid residues identical.
Additional Information
Gene ID 29413
Other Names Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-1D adrenoceptor, RA42, Adra1d, Adra1a
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)EPVPPDERF*SGITEE, corresponding to amino acid residues  231-245 of rat ־±1D-Adrenoceptor with replacement of cysteine 240  (C240) with serine (*S) (Accession P23944 ). 3rd extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Adrenoceptors (also called Adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. The Adrenoceptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.The Adrenoceptors are divided into three types: α1, α2 and β-Adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β31,2. The Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system1,2. α1-Adrenergic Receptors play an important role in the physiological response to epinephrine and norepinephrine, particularly in the cardiovascular system. All three cloned α1 receptors (α1A, α1B, and α1D) couple to Gq/11. While their cellular distribution is clear, their functional role is less so due to the lack of specific agonists/antagonists3. As these receptors lack specific agonists/antagonists knock-out mice studies have most significantly shed light on the cellular role of Adrenoceptors. Individual α1-Adrenoceptor knock-out mice do not display physical abnormalities in general. Similar knock-out studies indicate that all α1 subtypes seem to be involved in the regulation of blood pressure3. Also, studies indicate that α1D-Adrenoceptor seems to play an important role in aortic contraction4, renal function, nociception, basal locomotor activity3 as well as bladder contraction5. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of rat α1D-Adrenoceptor. Anti-α1D-Adrenoceptor (extracellular) antibody (#AG1377) can be used in western blot, indirect flow cytometry and immunohistochemical applications. It has been designed to recognize α1D-Adrenoceptor from mouse, rat and human samples.


2. Piascik, M. T .and Perez, D. M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
3. Chen, Z.J. and Minneman, K.P. (2005) Acta Pharmacol. Sin. 26, 1281.
4. Tanoue, A. et al. (2002) J. Clin. Invest. 109, 765.
5. Chen, Q. et al. (2005) J. Urol. 174, 370.

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$ 367.00
$ 475.00
Cat# AG1377-050
Availability: 2-3 weeks
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