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alpha1B-Adrenoceptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - alpha1B-Adrenoceptor (extracellular) Antibody AG1378-050
    Western blot analysis of α1B-adrenoreceptor in lysates of rat brain (lanes 1and 3), rat kidney (lanes 2 and 4) and GH3 cell line (lanes 5 and 6): 1,2,5. Anti-α1B-Adrenoceptor (extracellular) antibody (#AG1378), (1:200). 3,4,6. Anti-α1B-Adrenoceptor (extracellular) antibody, preincubated with the control peptide antigen.
  • ICC - alpha1B-Adrenoceptor (extracellular) Antibody AG1378-050
    Expression of α1B-Adrenoceptor in GH3 cells Immunocytochemical staining of GH3 cells with Anti-α1B-Adrenoceptor (extracellular) antibody (#AG1378), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). Nuclear staining of cells using the DNA dye Hoechst 33342 (blue).
  • FC - alpha1B-Adrenoceptor (extracellular) Antibody AG1378-050
    Indirect flow cytometry analysis of GH3 living cells:
    Black: Unstained cells.
    Green: Cells + Anti-α1B-Adrenoceptor (extracellular) antibody (#AG1378), (10 µg/5x105 cells).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35368
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 56836 Da
Homology Mouse - 14/15 amino acid residues identical; rat - 13/15 amino acid residues identical.
Additional Information
Gene ID 147
Other Names Alpha-1B adrenergic receptor, Alpha-1B adrenoreceptor, Alpha-1B adrenoceptor, ADRA1B
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KNANFTGPNQTSSNS, corresponding to amino acid residues 21-35 of human ־±1B-adrenoceptor (Accession P35368). Extracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and massspectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Adrenoceptors (also called Adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. The Adrenoceptors are members of the G-protein-coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus. The Adrenoceptors are divided into three types: α1, α2 and β adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3. 1,2 They are expressed in nearly all peripheral tissues and in the central nervous system.1,2 All α1-Adrenoceptors (α1-ARs) activate phospholipases C and A2.3 In addition to mobilizing intracellular calcium, the α1-ARs have also been shown to activate calcium influx via voltage-dependent and independent calcium channels.4 The α1B-Adrenoceptor shows the highest levels in the spleen, kidney, cerebellum, and fetal brain.5 α1B Adrenoceptor causes contraction of smooth muscle cells and thereby controls vascular tone, blood pressure, and accelerates the development of atherosclerosis.5 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of the human α1B- Adrenoceptor. Anti-α1B-Adrenoceptor (extracellular) antibody (#AG1378) can be used in western blot, immunocytochemistry and indirect flow cytometry applications. It has been designed to recognize α1B-Adrenoceptor from human, rat and mouse samples.


2. Piascik, M. T .and Perez, D. M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
3. Perez, D.M. et al. (1993) Mol Pharmacol 44, 78.
4. Minneman, K.P. (1988) Pharmacol. Rev. 40, 87.
5. SCHWINN, D.A. et al. (2004) Mayo Clin Proc. 79, 1423.

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$ 475.00
$ 575.00
Cat# AG1378-050
(40 western blots)
Availability: 2-3 weeks
Bulk Size
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