|Application ||WB, IHC|
|Calculated MW||43006 Da|
|Homology||Rat - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.|
|Other Names||Beta-3 adrenergic receptor, Beta-3 adrenoreceptor, Beta-3 adrenoceptor, Adrb3, Adrb3r, B3bar|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide DAEAQESH*SNPRC, corresponding to amino acid residues 180-192 of mouse ־²3-Adrenoceptor with replacement of cysteine 186 (C186) with serine (*S) (Accession P25962 ). 2nd extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Adrenoceptors (also called Adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. The Adrenoceptors are members of the G-protein-coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.The Adrenoceptors are divided into three types: α1, α2 and β-Adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3. 1,2 The Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.1,2 The β3-Adrenoceptor is insensitive to the commonly used ß-antagonists and has often been referred to as the 'atypical' ß-Adrenoceptor. Mouse β3-Adrenoceptor has two known splice variants, isoforms β3a-Adrenoceptor and β3b-Adrenoceptor. Both isoforms are expressed in white and brown adipose tissues.3 The β3b-Adrenoceptor is highly exspressed in brain. The β3- Adrenoceptor has been found to play a key role in the lipolytic action of catecholamines.4 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of the mouse β3-Adrenoceptors. The Anti-β3-Adrenoceptor (#AG1379) can be used in western blot and immunohistochemical applications. It has been designed to recognize both splice variants of β3-Adrenoceptor from mouse, rat and human samples.
1. IUPHAR RECEPTOR DATABASE | ADRENOCEPTORS
2. Piascik, M. T .and Perez, D. M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
3. Evans, B. A. et al. (1999) Br. J. Pharmacol. 127, 1525.
4. Klein, J. et al (1999) J.Biol.Chem. 274, 34795.
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