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beta3-Adrenoceptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

  • WB - beta3-Adrenoceptor (extracellular) Antibody AG1379-050
    Western blot analysis of mouse brain (lanes 1 and 3) and rat brain (lanes 2 and 4) lysates: 1, 3. Anti-β3-Adrenoceptor (extracellular) antibody (#AG1379), (1:200). 2, 4. Anti-β3-Adrenoceptor (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - beta3-Adrenoceptor (extracellular) Antibody AG1379-050
    Expression of β3-Adrenoceptor in rat hippocampus Immunohistochemical staining of rat hippocampal dentate gyrus using Anti-β3-Adrenoceptor antibody (#AG1379), (1:100), (green). β3-Adrenoceptor is strongly expressed in the hilus (H) and in the outer molecular layer (arrows). The distribution overlaps the entire layers, but is not restricted to nerve cells (stained red with mouse anti Parvalbumin). DAPI is used as the counterstain (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25962
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 43006 Da
Homology Rat - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.
Additional Information
Gene ID 11556
Other Names Beta-3 adrenergic receptor, Beta-3 adrenoreceptor, Beta-3 adrenoceptor, Adrb3, Adrb3r, B3bar
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide DAEAQESH*SNPRC, corresponding to amino acid residues 180-192 of mouse ־²3-Adrenoceptor with replacement of cysteine 186 (C186) with serine (*S) (Accession P25962 ). 2nd extracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.75 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Adrenoceptors (also called Adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. The Adrenoceptors are members of the G-protein-coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.The Adrenoceptors are divided into three types: α1, α2 and β-Adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3. 1,2 The Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.1,2 The β3-Adrenoceptor is insensitive to the commonly used ß-antagonists and has often been referred to as the 'atypical' ß-Adrenoceptor. Mouse β3-Adrenoceptor has two known splice variants, isoforms β3a-Adrenoceptor and β3b-Adrenoceptor. Both isoforms are expressed in white and brown adipose tissues.3 The β3b-Adrenoceptor is highly exspressed in brain. The β3- Adrenoceptor has been found to play a key role in the lipolytic action of catecholamines.4 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of the mouse β3-Adrenoceptors. The Anti-β3-Adrenoceptor (#AG1379) can be used in western blot and immunohistochemical applications. It has been designed to recognize both splice variants of β3-Adrenoceptor from mouse, rat and human samples.


2. Piascik, M. T .and Perez, D. M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
3. Evans, B. A. et al. (1999) Br. J. Pharmacol. 127, 1525.
4. Klein, J. et al (1999) J.Biol.Chem. 274, 34795.

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$ 367.00
$ 475.00
Cat# AG1379-050
Availability: 2-3 weeks
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