|Application ||WB, IHC|
|Calculated MW||46998 Da|
|Homology||Human - 15/16 amino acid residues identical; rat - 14/16 amino acid residues identical.|
|Other Names||Beta-2 adrenergic receptor, Beta-2 adrenoreceptor, Beta-2 adrenoceptor, Adrb2, Adrb2r|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)NGSRAPDHDVTQERDE, corresponding to amino acid residues 15-30 of mouse ־²2-Adrenoceptor (Accession P18762). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and massspectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.7 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Adrenoceptors (also called adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. The Adrenoceptors are members of the G-protein-coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.The Adrenoceptors are divided into three types: α1, α2 and β-Adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3. 1,2 The Adrenoceptors are expressed in nearly all peripheral tissues and in the central nervous system.1,2 β2-Adrenoceptors are mainly postsynaptic receptors. They are expressed on a number of tissues including blood vessels, bronchi, gastrointestinal tract (GIT), skeletal muscle, liver and mast cell.3 Functional β2-Adrenoceptors were also found on lymphocytes, macrophages and neutrophils.4,5 They are also the only β-Adrenoceptor subtype expressed in keratinocytes, fibroblasts and melanocytes.3 Activation of β2-Adrenoceptors results in vasodilation, relaxation of the GIT, inhibition of histamine release from mast cells and bronchodilation. Selective agonists of β2-Adrenoceptors are used to treat asthma and other related bronchospastic conditions such as Chronic obstructive pulmonary disease (COPD).5 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of the mouse β2-Adrenoceptor. Anti-β2-Adrenoceptor (extracellular) antibody (#AG1380) can be used in western blot and immunohistochemistry applications. It has been designed to recognize β2-Adrenoceptor from mouse, rat and human samples.
1. IUPHAR RECEPTOR DATABASE | ADRENOCEPTORS
2. Piascik, M. T .and Perez, D. M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
3. Pullar, C.E. et al. (2006) FASEB J. 20, 76.
4. Oostendorp, J. et al (2005) Am. J. Respir. Crit. Care Med. 172, 322.
5. Giembycz, M. A. and Newton, R. (2006) Eur. Respir. J. 27, 1286.
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