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alpha1A-Adrenoceptor (extracellular) Antibody

Affinity purified rabbit polyclonal antibody

     
  • WB - alpha1A-Adrenoceptor (extracellular) Antibody AG1381-050
    Western blot analysis of lysates from K562, PC-3 cell line (from left to right), using ADRA1A Antibody(Cat. #AG1381). AG1381 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - alpha1A-Adrenoceptor (extracellular) Antibody AG1381-050
    Western blot analysis of lysates from human brain tissue and K562 cell line (from left to right), using ADRA1A Antibody(Cat. #AG1381). AG1381 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • WB - alpha1A-Adrenoceptor (extracellular) Antibody AG1381-050
    Western blot analysis of PC3 cell line lysate (lanes 1 and 3) and rat brain membrane (lanes 2 and 4): 1, 2. Anti-α1A-Adrenoceptor (extracellular) antibody (#AG1381), (1:200). 3, 4. Anti-α1A-Adrenoceptor (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - alpha1A-Adrenoceptor (extracellular) Antibody AG1381-050
    Expression of α1A-Adrenoceptor in rat cerebellum Immunohistochemical staining of rat cerebellum using Anti-α1A-Adrenoceptor (extracellular) antibody (#AG1381), (1:100). A. α1A-Adrenoceptor (green) appears in fibers of Bergmann glia (triangle points at an example). B. S100β (red), a marker of Bergmann glia, is stained in the same section. C. Merge of the images demonstrates expression of α1A-Adrenoceptor in fibers of Bergmann glia. α1A-Adrenoceptor is also expressed in cells in the molecular layer that are s100ß negative (arrow points at an example). DAPI is used as the counterstain (blue).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession P35348
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 51487 Da
Homology Human, rat, mouse, bovine - 12/13 amino acid residues identical.
Additional Information
Gene ID 148
Other Names Alpha-1A adrenergic receptor, Alpha-1A adrenoreceptor, Alpha-1A adrenoceptor, Alpha-1C adrenergic receptor, Alpha-adrenergic receptor 1c, ADRA1A, ADRA1C
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide EDETI*SQINEEPG(C), corresponding to amino acid residues 171-183 of human ־±1A adrenoceptor with replacement of cysteine 176 (C176) with serine (*S) (Accession  P35348). 2nd extracellular loop.
Dilution WB~~1:200-1:2000
IHC~~1:100
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.70 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 2 µg peptide per 1 µg antibody.
Research Areas
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Background

Adrenoceptors (also called Adrenergic receptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions. The Adrenoceptors are members of the G-protein-coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus. The Adrenoceptors are divided into three types: α1, α2 and β adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3. 1,2 They are expressed in nearly all peripheral tissues and in the central nervous system.1,2 All α1-Adrenoceptors (α1-ARs) activate phospholipases C and A2.3 In addition to mobilizing intracellular calcium, the α1-ARs have also been shown to activate calcium influx via voltage-dependent and independent calcium channels.4 The α1A-ARs have at least nine known alternative spliced isoforms. They are the most abundant adrenergic receptor in the brain, and considered postsynaptic receptors. α1A-ARs are also expressed in heart, liver and prostate. Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of the human α1A-Adrenoceptors. Anti-α1A-Adrenoceptor (extracellular) antibody (#AG1381) can be used in western blot analysis and immunohistochemical applications. It has been designed to recognize α1A-Adrenoceptor from human, rat and mouse samples.

References

1. IUPHAR RECEPTOR DATABASE | ADRENOCEPTORS.
2. Piascik, M. T .and Perez, D. M. (2001) J. Pharmacol. Exp. Ther. 298, 403.
3. Perez, D.M. et al. (1993) Mol Pharmacol 44, 78.
4. Minneman, K.P. (1988) Pharmacol. Rev. 40, 87.

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$ 475.00
$ 575.00
Cat# AG1381-050
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