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>   home   >   Products   >   Primary Antibodies   >   Angiotensin-(1-7) Mas Receptor Antibody   

Angiotensin-(1-7) Mas Receptor Antibody

Affinity purified polyclonal antibody

     
  • WB - Angiotensin-(1-7) Mas Receptor Antibody AG1382-025
    Western blot analysis of rat brain (lanes 1 and 4) kidney (lanes 2 and 5) and heart (lanes 3 and 6) membranes: 1-3. Anti-Angiotensin-(1-7) Mas Receptor antibody (AG1382), (1:200).
    4-6. Anti-Angiotensin-(1-7) Mas Receptor, preincubated with the control peptide antigen.
  • WB - Angiotensin-(1-7) Mas Receptor Antibody AG1382-025
    Western blot analysis of mouse kidney membranes: 1. Anti-Angiotensin-(1-7) Mas Receptor antibody (AG1382), (1:200).
    2. Anti-Angiotensin-(1-7) Mas Receptor, preincubated with the control peptide antigen.
  • WB - Angiotensin-(1-7) Mas Receptor Antibody AG1382-025
    Western blot analysis of human HeLa cervix adenocarcinoma cell line lysate: 1. Anti-Angiotensin-(1-7) Mas Receptor antibody (AG1382),(1:200).
    2. Anti-Angiotensin-(1-7) Mas Receptor, preincubated with the control peptide antigen.
  • IHC - Angiotensin-(1-7) Mas Receptor Antibody AG1382-025
    Expression of Angiotensin-(1-7) Mas Receptor in rat kidney Immunohistochemical staining of rat kidney paraffin embedded sections using Anti-Angiotensin-(1-7) Mas Receptor antibody (#AG1382), (1:100). Angiotensin-(1-7) Mas receptor (brown staining) is detected in proximal tubules (PT) and distal tubules (DT) in the renal cortex. Collecting ducts (CD) are less stained and both glomeruli (G) and blood vessels (A) are negative.  Hematoxilin is used as the counterstain.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P12526
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 37131 Da
Homology Mouse, human - identical.
Additional Information
Gene ID 25153
Other Names Proto-oncogene Mas, Mas1, Mas, Mas-1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KIRKNTWASHSSK, corresponding to amino acid residues 212-224 of the rat Angiotensin-(1-7) Mas receptor (Accession P12526).ֲ 3rd intracellularֲ loop.
Dilution WB~~1:200-1:2000
IHC~~1:100
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Rat heart lysate (1:5000) (see Wang, H. et al. (2013) in Product Citations). - Mouse lung lysate (1:500) (see Rodriguez-Machado, M.G. et al. (2013) in Product Citations). - Sheep brain medulla lysate (1:250) (see Marshall, A.C. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Rat striatum (see Regenhardt, R.W. et al. (2014) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.55 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
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Background

The angiotensin-(1-7) Mas receptor is the recently identified receptor of the biologically active heptapeptide angiotensin-(1-7).1   Angiotensin (Ang)-(1-7) is a metabolite of the well known peptide hormone Angiotensin (Ang) II, a key component of the renin-angiotensin system (RAS) that has a central role in cardiovascular homeostasis.   Considerably interest in Ang-(1-7) and its receptor aroused in the last few years since it became apparent that it can counterbalance most of Ang II effects. Thus Ang-(1-7) has vasodilator and hypotensive effects as well as antiarrhythmic and cardioprotective roles.2, 3   The Ang-(1-7) Mas receptor belongs to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily and was originally described as a protooncogene. Signaling via the Ang-(1-7) Mas receptor is still poorly elucidated however, evidence indicates that the receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC).2,3   The Ang-(1-7) Mas receptor is expressed in several organs including heart, kidney, blood vessels, testis and brain. Studies with Ang-(1-7) Mas receptor knockout mice have demonstrated the key role of this receptor in cardiovascular regulation as well as in the regulation of learning and memory.2

References

References 1. Santos, R.A. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 8258. 2. Alenina, N. et al. (2008) Exp. Physiol. 93, 528. 3. Santos, R.A. et al. (2008) Exp. Physiol. 93, 519.

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Cat# AG1382-025
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