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A1 Adenosine Receptor Antibody

Affinity purified rabbit polyclonal antibody

  • WB - A1 Adenosine Receptor Antibody AG1383-050
    Western blot analysis of rat brain (lanes 1,3) and rat kidney (lanes 2,4) lysates: 1,2. Anti-A1 Adenosine Receptor antibody (#AG1383), (1:200). 3,4. Anti-A1 Adenosine Receptor antibody, preincubated with the control peptide antigen.
  • IHC - A1 Adenosine Receptor Antibody AG1383-050
    Expression of A1 Adenosine Receptor in rat cortex Immunohistochemical staining of rat cortex frozen section using Anti-A1 Adenosine Receptor antibody (#AG1383). A. A1 Adenosine Receptor (green) appears in neurons (triangles) and in astrocytes (arrows). B. Parvalbumin staining (red) appears in cortical interneurons. C. Confocal merge of images demonstrates the existence of A1 Adenosine Receptor in a subset of cortical interneurons and astrocytes.
  • IHC - A1 Adenosine Receptor Antibody AG1383-050
    Expression of A1 Adenosine Receptor in rat medial septum Immunohistochemical staining of rat medial septum frozen section using Anti-A1 Adenosine Receptor antibody (#AG1383). A. A1 Adenosine Receptor (green) appears in neurons (right pointing triangles). B. Parvalbumin staining (red) appears in medial septal neurons. C. Confocal merge of demonstrates expression of A1 Adenosine Receptor in a subset of medial septal neurons.
  • ICC - A1 Adenosine Receptor Antibody AG1383-050
    Expression of A1 Adenosine Receptor in rat DRG primary culture Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture. A. Staining of DRG cells with Anti-A1 Adenosine Receptor antibody (#AG1383), (1:100), followed by goat anti-rabbit AlexaFluor-555 secondary antibody. B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged images of A and B. F. Merged images of D and E. Magnification: A-C: x20 D-F: x100
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30542
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 36512 Da
Homology Mouse, rat, bovine, canis and pig - identical.
Additional Information
Gene ID 134
Other Names Adenosine receptor A1, ADORA1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KKVSASSGDPQKYYGKE, corresponding to amino acid residues 213-229 of human A1AR (Accession P30542). 3rd intracellular loop.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Mouse muscle, brain and spinal cord lysates (1:5000) (see Garcia, N. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Mouse plastic-embedded muscle sections (1:100) (see Garcia, N. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.85 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
Citations (0)

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Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including brain and heart.1,2It exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): A1-Adenosine Receptor (A1AR), A2A-Adenosine Receptor (A2AAR), A2B-Adenosine Receptor (A2BAR), and A3-Adenosine Receptor (A3AR). All are integral membrane proteins and members of the G Protein-Coupled Receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important in binding G proteins.1-3 The various adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3 A1AR is widely distributed and has been well characterized. High expression of A1AR is found in brain (mainly in the cortex, cerebellum, and hippocampus), dorsal horn of the spinal cord, adrenal gland, and atria, and to a lower extent in several other tissues including adipose tissue, colon, and kidney.2,4 A1AR modulates the activity of several ion channels. Activation of A1AR (by adenosine, its major agonist) inhibits N-type Ca2+ channels via a voltage-dependent, pertussis toxin (PTX)-sensitive pathway in neurons of the rat major pelvic ganglia (MPG).5 Since A1AR is the most prominent adenosine receptor in adipocytes, it has become a natural target for research on obesity, which is a major health problem.6,7 A possible role in cell proliferation and carcinogenesis has also been suggested for A1AR.8-10


1. Okusa, M.D. (2002) Am. J. Physiol. 282, F10.
2. Nakata, H. (1989) J. Biol. Chem. 264, 16545.
3. Linden, J. (1991) FASEB J. 5, 2668.
4. Park, K-S. et al. (2001) J. Pharmacol. Exp. Therap. 299, 501.
5. Rice, A.M. et al. (2000) Endocrinology 141, 1442.
6. LaNoue, K.F. and Martin, L.F. (1994) FASEB J. 8, 72.
7. Synowitz, M. et al. (2006) Cancer Res. 66, 8550.
8. Lelievre, V. et al. (1998) Eur. J. Pharmacol. 341, 289.
9. Khoo, H.E. et al. (1996) Cancer Lett. 106, 17.

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$ 475.00
$ 575.00
Cat# AG1383-050
(40 western blots)
Availability: 2-3 weeks
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