|Application ||WB, IHC, ICC|
|Homology||Rat, mouse - 14/16 amino acid residues identical.|
|Other Names||Adenosine receptor A3, ADORA3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KETGAFYGREFKTAK, corresponding to amino acid residues 216-230 of human A3AR (Accession P33765). 3rd intracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.7 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): A1-Adenosine receptor (A1AR), A2A-Adenosine receptor (A2AAR), A2B-Adenosine receptor (A2BAR), and A3-Adenosine receptor (A3AR). The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs.1-4 However, all are integral membrane proteins and members of the G Protein-Coupled Receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important for binding G proteins.1-3 Expression of A3AR has been reported in brain, kidney, liver, and heart. It plays a role in modulation of cerebral ischemia, asthma, and cell growth. In addition, recent studies have established a cardioprotective role for A3AR.5 Expression of A3AR was reported to be elevated in cancerous tissues as well as in auto-immune inflammatory diseases.6,7 A patent has been filed for the use of A3AR and A3AR agonist as a diagnostic marker for therapeutic treatments of cancer and other diseases. Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of human A3 Adenosine receptor (A3AR). Anti-A3 Adenosine Receptor antibody (#AG1384) can be used in western blot analysis, as well as immunocytochemical and immunohistocemical applications, and has been designed to recognize A3 Adenosine receptor from human, rat, and mouse samples.
1. Okusa, M.D. (2002) Am. J. Physiol. Renal Physiol. 282, F10.
2. Fredholm, B.B. et al. (2001) Pharmacol. Rev. 53, 527.
3. Nakata, H. (1989) J. Biol. Chem. 264, 16545
4. Baraldi, P.G. et al. (2006) Curr. Med. Chem. 13, 3467.
5. Headrick, J.P. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 285, H1797.
6. Madi, L. et al. (2004) Clin. Cancer Res. 10, 4472.
7. Ochaion, A. et al. (2007) Ann. Rheum. Dis. 66, 446.
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