|Application ||WB, IHC, FC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||36333 Da|
|Homology||Rat - 14/19 amino acid residues identical; mouse - 13/19 amino acid residues identical.|
|Other Names||Adenosine receptor A2b, ADORA2B|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide KDSATNN*STEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2B Adenosine Receptor with replacement of cysteine 154 (C154) with serine (*S) (Accession P29275). 2nd extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): A1-Adenosine Receptor (A1AR), A2A-Adenosine Receptor (A2AAR), A2B-Adenosine Receptor (A2BAR), and A3-Adenosine Receptor (A3AR). All are integral membrane proteins and members of the G Protein-Coupled Receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important in binding G proteins.1-3 The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs.1-4 The A2 Adenosine receptor subtype was subdivided according to affinity for adenosine; A2AAR has high affinity and A2BAR has low affinity. A2BAR is expressed in mast cells, neutrophils, monocytes, macrophages, dendritic cells, T cells, bronchial epithelium, intestinal epithelium, smooth muscle, brain, and other cells.5 In intestinal epithelia, A2BAR was found to be the major adenosine receptor subtype expressed and is thought to be involved in diarrheal diseases. It is also believed to be involved in the pathogenesis of chronic airway inflammatory diseases.4 Thus, antagonists of A2BAR might be effective for the treatment of inflammatory gastrointestinal tract disorders4 as well as asthma and chronic obstructive pulmonary disease.5 Abgent is pleased to offer a highly specific antibody directed against an extracellular epitope of the human A2B Adenosine receptor (A2BAR). The Anti-A2B-Adenosine Receptor (extracellular) antibody (#AG1385) can be used for western blot immunohistochemical applications. It has been designed to recognize A2B-Adenosine receptor from human, rat and mouse samples.
1. Okusa, M.D. (2002) Am. J. Physiol. Renal Physiol. 282, F10.
2. Fredholm, B.B. et al. (2001) Pharmacol. Rev. 53, 527.
3. Nakata, H. (1989) J. Biol. Chem. 264, 16545.
4. Baraldi, P.G. et al. (2006) Curr. Med. Chem. 13, 3467.
5. Polosa, R. (2002) Eur. Respir. J. 20, 488.
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