|Calculated MW||90045 Da|
|Homology||Rat, mouse - 13/14 amino acid residues are identical.|
|Other Names||Transmembrane channel-like protein 6, Epidermodysplasia verruciformis protein 1, Protein LAK-4, TMC6, EVER1, EVIN1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CGPFRTLDTMYEAGR, corresponding to amino acid residues 681-695 of human TMC6 (Accession Q7Z403). Intracellular, Cytoplasm.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.85 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
EVER gene belongs to larger family of proteins referred to as the transmembrane channel-like protein (TMC) family1. EVER1/TMC6 protein, integral membrane protein, is located in the endoplasmic reticulum (ER) of keratinocytes, where it forms a complex with zinc transporter-1 (ZnT-1) a membrane protein responsible for zinc efflux and resistance to zinc toxicity, thereby controlling the cellular zinc balance2. The EVER1 protein is predicted to have 10 transmembrane domains and 2 leucine zipper motifs1. The EVER1 gene encodes 2 alternatively spliced proteins, one of 805 amino acids and the other of 454 amino acids, in the same open reading frame. The EVER genes are thought to be transcribed in numerous tissues including the skin1. Mutations in the TMC6 are implicated in the development of the rare autosomal recessive disease Epidermodysplasia Verruciformis (EV)3. EV is characterized by extreme susceptibility to infection by cutaneous human papillomaviruses (HPV) of the beta genus. The TMC6 genes may be involved in controlling HPV gene expression and replication in epidermal keratinocytes or they may directly affect innate and adaptive immune responses which control the clearance of HPV-infected keratinocytes in EV cases4. A recent report indicated an association between genetic variation in the TMC6 region and development of cervical cancer (CxCa) 5.
References 1. Lazarczyk, M. et al. (2009) Microbiol. Mol. Biol. Rev. 73, 348. 2. Lazarczyk, M. et al. (2008) J. Exp. Med. 205, 35. 3. Ramoz, N. et al. (2002) Nat. Genet. 32, 579. 4. Orth, G. et al. (2008) Curr. Top Microbiol. Immunol. 321, 59. 5. Castro, F.A. et al. (2012) Int. J. Cancer 130, 349.
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