|Calculated MW||59641 Da|
|Homology||Human - identical; mouse- 19/20 amino acid residues identical.|
|Other Names||Acid-sensing ion channel 1, ASIC1, Amiloride-sensitive cation channel 2, neuronal, Brain sodium channel 2, BNaC2, Asic1, Accn2, Bnac2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1ֲ (accession number P55926). Intracellular, C-terminus.Recognizes isoforms ASIC1־± and ASIC1ֳ.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
ASIC1 is a member of a family of Na+ channels that are activated by external protons. The family includes another three members ASIC2, ASIC3 and ASIC4. The ASICs are in fact part of a larger superfamily termed epithelial Na+ channels (EnaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini. There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions. The ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium. Lately, ASIC1 has been implicated in processes such as learning and memory in the central nervous system. Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of rat ASIC1. The Anti-ASIC1 antibody (#AG1389) can be used in western blot and immunohistochemical applications. It has been designed to recognize ASIC1 from rat, human and mouse samples, and recognizes both ASIC1 isoforms.
References 1. Kellenberger, S. et al. (2002) Physiol. Rev. 82, 735. 2. Wemmie, J. A. et al. (2002) Neuron 34, 463. 3. Wemmie, J. A. et al. (2003) J. Neurosci. 23, 5496.
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