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NaV1.6 Antibody

Affinity purified polyclonal antibody

  • WB - NaV1.6 Antibody AG1390-025
    Western blot analysis of rat brain membrane: 1. Anti-Nav1.6 antibody (#AG1390), (1:200).
    2. Anti-Nav1.6 antibody, preincubated with the control peptide antigen.
  • IHC - NaV1.6 Antibody AG1390-025
    Expression of Nav1.6 in mouse hippocampus
    Immunohistochemical staining of mouse hippocampus using Anti-Nav1.6 antibody (#AG1390). A. Nav1.6 (green) is robustly expressed in the CA1 pyramidal layer (white arrows). B. Staining with mouse anti-parvalbumin (red), a marker of interneurons. C. merged image of panels A and B reveals that Nav1.6 appears in some interneurons (arrow) but is not restricted to interneurons. DAPI is used as the counterstain.
  • ICC - NaV1.6 Antibody AG1390-025
    Expression of Nav1.6 in rat DRG primary culture
    Immunocytochemical staining of paraformaldehyde-fixed and permeabilized DRG primary culture. A. Staining using Anti-Nav1.6 antibody (#AG1390), (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. B. Nuclear staining using the cell-permeable dye Hoechst  33342. C. Merged image of panels A and B.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O88420
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 225159 Da
Homology Mouse - identical; human - 19/20 amino acid residues identical.
Additional Information
Gene ID 29710
Other Names Sodium channel protein type 8 subunit alpha, Peripheral nerve protein type 4, PN4, Sodium channel 6, NaCh6, Sodium channel protein type VIII subunit alpha, Voltage-gated sodium channel subunit alpha Nav16, Scn8a
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CIANHTGVDIHRNGDFQKNG, corresponding to amino acid residues 1042-1061 of rat Nav1.6 (Accession O88420).ֲ ֲ Intracellular loop between domains II and III.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Application Details Western blot analysis (WB): - Immortalized mouse microglial cells (BV-2) (1:500) (see Hossain, M.M. et al. (2013) in Product Citations). - Human and Mouse brain (see Verret, L. et al. (2012) in Product Citations). - Mouse brain lysate (see Kearney, J.A. et al. (2002) in Product Citations).   Immunohistochemistry (IH): - Mouse spinal cord and cortex (1:100) (see Zoupi, L. et al. (2013) in Product Citations). - Rat brain sections (1:250) (see Hargus, N.J. et al. (2013) in Product Citations). - Rat DRG sections (1:100) (see Xie, W. et al. (2013) in Product Citations). Also tested in NaV1.6 siRNA treated animals. - Human cervical tissue (1:25) (see Hernandez-Plata, E. et al. (2012) in Product Citations). - Mouse brain sections (see Takano, M. et al. (2012) in Product Citations). - Mouse DRG sections (see Wittmack, E.K. et al. (2004) in Product Citations). - Mouse heart sections (see Maier, S.K.G. et al. (2003) in Product Citations). - Rat heart sections (see Maier, S.K.G. et al. (2003) in Product Citations). - Rat optic nerve tissue (see Jenkins, S.M. and Bennet, V. (2002) in Product Citations).   Immunocytochemistry (IC): - Immortalized mouse microglial cells (BV-2) (1:250) (see Hossain, M.M. et al. (2013) in Product Citations). - Human cervical cancer cell line (1:25) (see Hernandez-Plata, E. et al. (2012) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5  The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7)  and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. Expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7   NaV1.6 is highly expressed in adult brain and localized at high density in nodes of Ranvier and axon initial segments and at lower density in dendrites and cell bodies of some neurons. NaV1.6 channels are also expressed at high levels in cerebellar Purkinje neurons.8-11


References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. 3. Fjell, J. et al. (2000) NeuroReport 11, 199. 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27 5. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. 6. Isom, L.L. (2001) Neuroscientist  7, 42. 7. Catterall, W.A. et al. (2003) Pharmacol. Rev  55, 575. 8. Catterall, W.A. et al. (2005) Pharmacol Rev. 57, 397. 9. Boiko, T. et al. (2001) Neuron 30, 91. 10. Caldwell, J.H. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 5616. 11. Raman I. M and Bean B.P. (1997) J. Neurosci. 17, 4517

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Cat# AG1390-025
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