|Reactivity||Human, Mouse, Rat|
|Calculated MW||225159 Da|
|Homology||Mouse - identical; human - 19/20 amino acid residues identical.|
|Other Names||Sodium channel protein type 8 subunit alpha, Peripheral nerve protein type 4, PN4, Sodium channel 6, NaCh6, Sodium channel protein type VIII subunit alpha, Voltage-gated sodium channel subunit alpha Nav16, Scn8a|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CIANHTGVDIHRNGDFQKNG, corresponding to amino acid residues 1042-1061 of rat Nav1.6 (Accession O88420).ֲ ֲ Intracellular loop between domains II and III.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Immortalized mouse microglial cells (BV-2) (1:500) (see Hossain, M.M. et al. (2013) in Product Citations). - Human and Mouse brain (see Verret, L. et al. (2012) in Product Citations). - Mouse brain lysate (see Kearney, J.A. et al. (2002) in Product Citations). Immunohistochemistry (IH): - Mouse spinal cord and cortex (1:100) (see Zoupi, L. et al. (2013) in Product Citations). - Rat brain sections (1:250) (see Hargus, N.J. et al. (2013) in Product Citations). - Rat DRG sections (1:100) (see Xie, W. et al. (2013) in Product Citations). Also tested in NaV1.6 siRNA treated animals. - Human cervical tissue (1:25) (see Hernandez-Plata, E. et al. (2012) in Product Citations). - Mouse brain sections (see Takano, M. et al. (2012) in Product Citations). - Mouse DRG sections (see Wittmack, E.K. et al. (2004) in Product Citations). - Mouse heart sections (see Maier, S.K.G. et al. (2003) in Product Citations). - Rat heart sections (see Maier, S.K.G. et al. (2003) in Product Citations). - Rat optic nerve tissue (see Jenkins, S.M. and Bennet, V. (2002) in Product Citations). Immunocytochemistry (IC): - Immortalized mouse microglial cells (BV-2) (1:250) (see Hossain, M.M. et al. (2013) in Product Citations). - Human cervical cancer cell line (1:25) (see Hernandez-Plata, E. et al. (2012) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 NaV channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.4-5 The NaV channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9).2-3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. Expression of the α subunit isoform is developmentally regulated and tissue specific. Sodium channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas sodium channels in adult skeletal muscle have only the β1 subunit.6,7 NaV1.6 is highly expressed in adult brain and localized at high density in nodes of Ranvier and axon initial segments and at lower density in dendrites and cell bodies of some neurons. NaV1.6 channels are also expressed at high levels in cerebellar Purkinje neurons.8-11
References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. 3. Fjell, J. et al. (2000) NeuroReport 11, 199. 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27 5. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. 6. Isom, L.L. (2001) Neuroscientist 7, 42. 7. Catterall, W.A. et al. (2003) Pharmacol. Rev 55, 575. 8. Catterall, W.A. et al. (2005) Pharmacol Rev. 57, 397. 9. Boiko, T. et al. (2001) Neuron 30, 91. 10. Caldwell, J.H. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 5616. 11. Raman I. M and Bean B.P. (1997) J. Neurosci. 17, 4517
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