|Reactivity||Human, Mouse, Rat|
|Calculated MW||24145 Da|
|Homology||Human - 17/19 amino acid residues identical.|
|Other Names||Sodium channel subunit beta-2, Scn2b|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KTEEEGKTDGEGNAEDGAK, corresponding to amino acid residues 197-215 of rat ־²2 subunit of voltage-gated Na+ channels (Accessionֲ P54900). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Human and mouse cortical lysates (see Corbett, B.F. et al. (2013) in Product Citations). - Canine midmyocardial ventricular cardiomyocytes (VCMs) (see Mishra, S. et al. (2011) in Product Citations). - Neonatal rat cardiomyocytes (1:200) (see Kang, L. et al. (2009) in Product Citations). Immunohistochemistry (IH): - Mouse cortical sections (see Corbett, B.F. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.2,3 Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β-subunits (NaVβ-subunit).4 The NaV β-subunit gene family consists of four members: β1 (SCN1B), β2 (SCN2B), β3 (SCN3B), and β4 (SCN4B) having type I topology, containing an extracellular amino-terminus, a single transmembrane segment, and an intracellular carboxyl-terminus.They modulate channel gating, assembly, and cell surface expression in heterologous cell systems.5 NaV β-subunits are cell adhesion molecules of the Ig superfamily, which interact with extracellular matrix, transmembrane signaling, and cell adhesion molecules.5 In the adult central nervous system and heart, sodium channels are associate with β1- β4 subunits, whereas in adult skeletal muscle they are associate only with the β1 subunit.4,5 This association appears to be a late event in sodium channel biosynthesis.6 Expression of β2-subunits increases in sensory neurons following nerve injury. β2 deficient mice develop less mechanical allodynia in compare to wild type mice, suggesting a role for β2 subunit in pain sensation. 7
References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. 3. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. 4. Catterall, W. A. (2000) Neuron 26, 13. 5. Chen, C. et al. (2002) Proc. Natl. Acad. Sci. U.S.A. 99, 17072. 6. Schmidt, J. W., and Catterall W. A. (1986) Cell 46, 437. 7. Lopez-Santiago, L, F. (2006) The Journal of Neuroscience 6, 7984.
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