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>   home   >   Products   >   Primary Antibodies   >   Neuroscience   >   Nabeta2 Antibody   

Nabeta2 Antibody

Affinity purified polyclonal antibody

     
  • WB - Nabeta2 Antibody AG1391-025
    Western blot analysis of rat brain membranes under reducing (lanes 1 and 2) or non-reducing (lanes 3 and 4) conditions:*
    1,3.  Anti-Navβ2 antibody (#AG1391), (1:200).
    2,4.  Anti-Navβ2 antibody, preincubated with the control peptide antigen.
     * Navβ2 is disulfide-linked to the α subunit of voltage-gated Na+ channels (Schmidt, J.W., and Catterall, W.A. (1986) Cell 46, 437.)
  • IHC - Nabeta2 Antibody AG1391-025
    Expression of Navβ2 in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-Navβ2 antibody (#AG1391). A. Navβ2 channel (red) appears in Purkinje cells (vertical arrows) and in small cells in the molecular layer (horizontal arrows, Mol). B. Staining of Purkinje nerve cells with mouse anti-calcium binding protein (CBD28k, green) demonstrates the restriction of Navβ2 to cell bodies. C, Confocal merge of Navβ2 and CBD28k.
  • IHC - Nabeta2 Antibody AG1391-025
    Expression of Navβ2 in rat cerebellum
    Immunohistochemical staining of rat cerebellum using Anti-Navβ2 antibody (#AG1391). A. Navβ2 channel (red) appears in Purkinje cells (horizontal arrows) and is distributed diffusely in  the molecular layer (Mol). B. Staining of Purkinje nerve cells with mouse anti-calcium binding protein (CBD28k, green) demonstrates the restriction of Navβ2 to cell bodies. C. Confocal merge of Navβ2 and CBD28k.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P54900
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 24145 Da
Homology Human - 17/19 amino acid residues identical.
Additional Information
Gene ID 25349
Other Names Sodium channel subunit beta-2, Scn2b
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KTEEEGKTDGEGNAEDGAK, corresponding to amino acid residues 197-215 of rat ־²2 subunit of voltage-gated Na+ channels (Accessionֲ  P54900). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Human and mouse cortical lysates (see Corbett, B.F. et al. (2013) in Product Citations). - Canine midmyocardial ventricular cardiomyocytes (VCMs) (see Mishra, S. et al. (2011) in Product Citations). - Neonatal rat cardiomyocytes (1:200) (see Kang, L. et al. (2009) in Product Citations). Immunohistochemistry (IH): - Mouse cortical sections (see Corbett, B.F. et al. (2013) in Product Citations).
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.75 mg/ml.
Buffer After Reconstitution
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
Citations (0)

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Background

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability.1 To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.9.2,3  Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β-subunits (NaVβ-subunit).4   The NaV β-subunit gene family consists of four members: β1 (SCN1B), β2 (SCN2B), β3 (SCN3B), and β4 (SCN4B) having type I topology, containing an extracellular amino-terminus, a single transmembrane segment, and an intracellular carboxyl-terminus.They modulate channel gating, assembly, and cell surface expression in heterologous cell systems.5   NaV β-subunits are cell adhesion molecules of the Ig superfamily, which interact with extracellular matrix, transmembrane signaling, and cell adhesion molecules.5 In the adult central nervous system and heart, sodium channels are associate with β1- β4 subunits, whereas in adult skeletal muscle they are associate only with the β1 subunit.4,5 This association appears to be a late event in sodium channel biosynthesis.6   Expression of β2-subunits increases in sensory neurons following nerve injury. β2 deficient mice develop less mechanical allodynia in compare to wild type mice, suggesting a role for β2 subunit in pain sensation. 7

References

References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. 3. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. 4. Catterall, W. A. (2000) Neuron 26, 13. 5. Chen, C. et al. (2002) Proc. Natl. Acad. Sci. U.S.A.  99, 17072. 6. Schmidt, J. W., and Catterall W. A. (1986) Cell 46, 437. 7. Lopez-Santiago, L, F. (2006) The Journal of Neuroscience 6, 7984.

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