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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NaV1.1 Antibody   

NaV1.1 Antibody

Affinity purified polyclonal antibody

     
  • WB - NaV1.1 Antibody AG1393-025
    Western blot analysis of rat brain membranes:
    1. Anti-Nav1.1 antibody (#AG1393), (1:200).
    2. Anti-Nav1.1 antibody, preincubated with the control peptide antigen.
  • IHC - NaV1.1 Antibody AG1393-025
    Expression of Nav1.1 in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-Nav1.1 antibody (#AG1393). A. The distribution of Nav1.1 (red) forms a band (arrows) in  the molecular layer (Mol), close to the Purkinje cell bodies. B. Purkinje nerve cells are stained with mouse anti-Parvalbumin (green). C. Confocal merge of Nav1.1 and Parvalbumin.
  • ICC - NaV1.1 Antibody AG1393-025
    Expression of Nav1.1 in rat DRG cells  Immunocytochemical staining of Paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) using Anti-Nav1.1 antibody (#AG1393), (1:200), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. Nuclear staining of cells using the cell-permeable dye Hoechst 33342.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP
Primary Accession P04774
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 228770 Da
Homology Human - identical.
Additional Information
Gene ID 81574
Other Names Sodium channel protein type 1 subunit alpha, Sodium channel protein brain I subunit alpha, Sodium channel protein type I subunit alpha, Voltage-gated sodium channel subunit alpha Nav11, Scn1a
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)TASEHSREPSAAGRLSD, corresponding to amino acid residues 465-481 of rat Nav1.1 (Accession P04774).ֲ ֲ Intracellular loop between domains I and II.
Dilution WB~~1:200-1:2000
ICC~~1:200
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Application Details Western blot analysis (WB): - Human and mouse cortical lysates (see Corbett, B.F. et al. (2013) in Product Citations). - Human and Mouse brain (see Verret, L. et al. (2012) in Product Citations). - Mouse heart (see Haufe, V. et al. (2005) in Product Citations). - Mouse cardiac myocytes (see Malhotra, J.D. et al. (2001) in Product Citations). - Mouse brain (see Planells-Cases, R. et al. (2000) in Product Citations).   Immunoprecipitation (IP): - Mouse heart (see Haufe, V. et al. (2005) in Product Citations). - Mouse cardiac myocytes (see Malhotra, J.D. et al. (2001) in Product Citations).   Immunohistochemistry (IH): - Rat brain sections (1:250) (see Hargus, N.J. et al. (2013) in Product Citations). - Human brain (1:400) (see Wang, W. et al. (2011) in Product Citations). - Mouse heart (see Lei, M. et al. (2004) in Product Citations). - Mouse heart (see Malhotra, J.D. et al. (2001) in Product Citations). - Human and Mouse brain (see Verret, L. et al. (2012) in Product Citations).   Immunocytochemistry (IC): - Mouse LVA myocytes (1:100) (see Ednie, A.R. et al. (2013) in Product Citations). - Mouse heart (see Haufe, V. et al. (2005) in Product Citations). - Mouse heart (see Lei, M. et al. (2004) in Product Citations). - Mouse brain (see Planells-Cases, R. et al. (2000) in Product Citations). - Mouse brain (see Zhou, D. et al. in Product Citations). - Rat brain (see Black, J.A. et al. (2004) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.6 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
Citations (0)

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Background

Voltage-gated sodium channels (Nav) are essential for the generation of action potentials and for cell excitability1. Nav channels are activated in response to depolarization and selectively allow flow of Na+ ions. To date, nine Nav a subunits have been cloned and named Nav1.1-Nav1.94-5.  The Nav channels are classified into two groups according to their sensitivity to Tetrodotoxin (TTX): TTX-sensitive (Nav1.1, Nav1.2, Nav1.3, Nav1.4, Nav1.6 and Nav1.7)  and TTX-resistant (Nav1.5, Nav1.8 and Nav1.9)2-3. Mammalian sodium channels are heterotrimers, composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,7.   Nav1.1 is a highly tetrodotoxin-sensitive channel and is broadly expressed in neurons7.   Mutations in NaV1.1 are associated with at least two forms of epilepsy. Gain-of-function missense mutations are a primary cause of generalized epilepsy with febrile seizures plus (GEFS+). Loss-of-function mutations cause severe myoclonic epilepsy of infancy (SMEI) 8,9.

References

References 1. Wu, L. et al. (2002) NeuroReport 13, 2547. 2. Fang, X. et al. (2002) J. Neurosci. 22, 7425. 3. Fjell, J. et al. (2000) NeuroReport 11, 199. 4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27. 5. Lai, J. et al. (2003) Curr.Opin. Neurobiol 13, 291. 6. Isom, L.L. (2001) Neuroscientist  7, 42. 7. Catterall, W.A. et al. (2003) Pharmacol Rev  55, 575. 8. Catterall, W.A. et al. (2008) J. Neurosci. 28, 11768. 9. Rhodes, T.H. et al. (2004)  Proc. Natl. Acad. Sci. U.S.A. 101, 11147.

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