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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Ryanodine Receptor 2 Antibody   

Ryanodine Receptor 2 Antibody

Affinity purified polyclonal antibody

  • WB - Ryanodine Receptor 2 Antibody AG1394-025
    Western blot analysis of rat heart membranes:
    1. Anti-Ryanodine Receptor 2 antibody (#AG1394), (1:200).
    2. Anti-Ryanodine Receptor 2 antibody, preincubated with the control peptide antigen.
  • IHC - Ryanodine Receptor 2 Antibody AG1394-025
    Expression of Ryanodine Receptor 2  in rat cardiac muscle Immunohistochemical staining of paraffin-embedded sections of rat myocardium using Anti-Ryanodine Receptor 2 antibody (#AG1394), (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxilin is used as the counterstain.
  • IHC - Ryanodine Receptor 2 Antibody AG1394-025
    Expression of Ryanodine Receptor 2 in mouse cerebellum  Immunohistochemical staining of mouse cerebellum frozen sections with Anti-Ryanodine Receptor 2 antibody (#AG1394), (1:100), (green fluorescence). A. The highest expression of Ryanodine Receptor 2 is in the molecular layer (Asterisk) but there is also some expression in the soma of Purkinje cells (arrows). B. In the same section, there is staining for parvalbumin (red), a marker for Purkinje cells. C. Merged image of panels A and B demonstrates that Ryanodine Receptor 2 is localized both in the area surrounding the dendritic tree and in the soma of Purkinje cells. DAPI is used as the counterstain (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92736
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 564567 Da
Homology Rat, mouse - identical.
Additional Information
Gene ID 6262
Other Names Ryanodine receptor 2, RYR-2, RyR2, hRYR-2, Cardiac muscle ryanodine receptor, Cardiac muscle ryanodine receptor-calcium release channel, Type 2 ryanodine receptor, RYR2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CAGESMSPGQGRNN, corresponding to amino acid residues 1489-1502 of human Ryanodine Receptor 2 (Accession Q92736). Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis and mass-spectrography.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2  The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells. The ER and SR contain two Ca2+ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).3 The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).3 The Ryanodine receptors are homotetrameric proteins. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability.   RyR2 serves as an intracellular Ca2+ channel in the SR membrane. It is predominantly expressed in cardiac muscle where it plays a central role in cardiac excitation-contraction coupling. RyR2 is also expressed in the brain. 1-4   Abgent is pleased to offer a highly specific antibody directed against an epitope located at the cytoplasmic region of the human Ryanodine receptor 2 (RyR2). The Anti-Ryanodine Receptor 2 antibody (#AG1394) can be used in western blot analysis and Immunohistochemical applications. It has been designed to recognize RyR2 from human, rat and mouse samples.


References 1. Chakrabarti, R. And Chakrabarti, R. (2006) J.Cell. Biochem. 99, 1503. 2. Eisner, D.A. et al. (2005) Exp.Physiol. 90, 3. 3. Bers DM. (2004) J.Mol. Cell. Cardiol. 37, 417. 4. Fill, M. and Copello, J.A. (2002) Physiol Rev. 82, 893.

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$ 375.00
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$ 575.00
Cat# AG1394-025
(40 western blots)
Availability: 2-3 weeks
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