|Calculated MW||30369 Da|
|Homology||Mouse - 11/12 amino acid residues identical.|
|Other Names||Aquaporin-11, AQP-11, Aqp11|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)SLSLTKYHFDER, corresponding to amino acid residues 140- 151 of rat Aquaporin 11 (Accession Q8CHM1). 2nd extracellular loop.Not recommended for human samples.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The aquaporins (AQP), also known as the major intrinsic protein superfamily (MIP), are a family of integral membrane proteins. In mammals, 13 aquaporins were described (AQP0 – AQP12). Aquaporin 11 (AQP11) is a newly described member of the aquaporins family. Aquaporin-11 (AQP-11) has been identified with unusual pore-forming NPA (asparagine-proline-alanine) boxes. AQP-11 presents a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1. There are two subfamilies: the aquaporins, which transport only water and the aquaglyceroporins, which transport glycerol, urea, and other small solutes in addition to water2. Aquaporins and aquaglyceroporins are ubiquitously expressed, including in the plasma membrane of renal epithelia, brain astrocytes, and red blood cells3. AQP-11 is expressed in several tissues including testis, kidney, liver, and brain. In the brain, AQP11 is found in Purkinje cells, hippocampal neurons of CA1 and CA2, and cerebral cortical neurons4. In the kidney AQP-11 has a central role in vesicular homeostasis as demonstrated in experiments using AQP-11 knockout mice which display growth retardation and progressively die before weaning. The cause of death seems to be advanced renal failure5.
References 1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687. 2. Borgnia, M. et al. (1999) Annu Rev Biochem 68, 425. 3. Yasui, M. et al. (1999) Proc Natl Acad Sci, 96, 5808. 4. Gorelick, D.A. et al. (2006) BMC Biochem. 7, 14. 5. Morishita, Y. et al. (2005) Mol. Cell Biol. 25, 7770.
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