|Calculated MW||66872 Da|
|Homology||Mouse - identical; human - 13/15 amino acid residues identical; rabbit - 14/15 amino acid residues identical.|
|Other Names||PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, TPR-containing Rab8b-interacting protein, Tetratricopeptide repeat-containing Rab8b-interacting protein, Pex5Rp, TRIP8b, Pex5l, Pex2, Pex5r, Trip8b|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide(C)EKWDDVKFHGDRTSK, corresponding to amino acid residues 151-165 of rat TRIP8b (Accession Q925N3).|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
PEX5R (also called TRIP8b, tetratricopetide repeat-containing Rab8b-interacting protein) is a cytoplasmic protein expressed as a family of alternatively spliced isoforms. PEX5R /TRIP8b isoforms contain a large constant domain preceded by a variable region. The C-terminal half of TRIP8b comprises a tetratricopeptide repeat (TPR) protein binding domain1. Analysis of the Pex5p-like protein revealed the presence of a tetratricopeptide repeat (TPR) domain in its C-terminal half consisting of seven TPR motifs. Pex5R is almost exclusively expressed in brain2. Recent studies provide strong evidence that PEX5R interacts with the carboxyl-terminal region of Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and regulates their cell-surface expression level and cyclic nucleotide dependence3,4. HCN channels are key modulators of neuronal activity by providing the depolarizing cation current involved in rhythmogenesis, dendritic integration, and synaptic transmission. In contrast, Gosh et al. have shown that human PEX5R can bind to peroxisomal targeting signal-1 (PTS1) ligands5. Furthermore it was shown that enzyme alanine–glyoxylate aminotransferase (AGT) is recognized by PEX5R in the cytoplasm, which allows its subsequent translocation into the peroxisome6. TRIP8b may play a role in both normal neuronal function and in aberrant neuronal excitability associated with neurological diseases7.
References 1. Fransen, M. et al. (2008) Biochim. Biophys. Acta 1783, 864. 2. Maynard, E.L. et al. (2004) Proteins 55, 856. 3. Lewis, A.S. et al. (2009) J. Neurosci. 29, 6250. 4. Santoro, B. et al. (2004) J. Neurosci. 24, 10750. 5. Ghosh, D. and Berg, J.M. (2010) J. Am. Chem. Soc. 132, 3973. 6. Fodor, K. et al. (2012) PLoS Biol. 10, e1001309. 7. Lewis, A.S. et al. (2009) J. Neurosci. 29, 6250.
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