|Application ||WB, FC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||39439 Da|
|Homology||Rat - identical; mouse - 12/14 amino acid residues identical.|
|Other Names||P2Y purinoceptor 12, P2Y12, ADP-glucose receptor,|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KTTRPFKTSNPKNLLGAK, corresponding to amino acid residues 125-142 of human P2Y12 receptor. (Accession Q9H244). Intracellular, 2nd Cytoplasmic loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1 The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4 P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5 Abgent is please to offer a highly specific antibody directed against an extracellular epitope of human P2Y1 receptor. Anti-P2Y1 Receptor (extracellular) antibody (#AG1398) can be used in western blot analysis and indirect flow cytometry applications, and has been designed to recognize P2Y1 from mouse, rat and human samples.
References 1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol. 40, 841. 2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther. 307, 809. 3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev. 50, 413. 4. Abbracchio, M.P. et al. (2003) Trends. Pharmacol. Sci. 24, 52. 5. Jin, J. et al. (1998) J. Biol. Chem. 273, 2030.
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