|Reactivity||Human, Mouse, Rat|
|Calculated MW||44392 Da|
|Homology||Mouse- identical; human - 13/15 amino acid residues identical.|
|Other Names||P2X purinoceptor 3, P2X3, ATP receptor, Purinergic receptor, P2rx3|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)VEKQSTDSGAYSIGH, corresponding to amino acid residues 383-397 of rat P2X3 Receptorֲ (Accession P49654).ֲ ֲ Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Mouse neuron lysate (1:300) (see Gnanasekaran, A. et al. (2013) in Product Citations.). Immunocytochemistry (IC): - Mouse trigeminal neurons (1:200) (see Gnanasekaran, A. et al. (2013) in Product Citations.). - Fixed mouse trigeminal neurons (1:200) (see D'Arco, M. et al. (2007) in Product Citations.). Indirect flow cytometry (IFC): - Human CD4+ and ckit+ cells (see Kazakova, R.R. et al. (2011) in Product Citations.).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
The P2X3 receptor belongs to the ligand-gated ion channel P2X family, that consists of seven receptor subtypes named P2X1-P2X7 and is activated by extracellular ATP. 1,2,3 All P2X subunits, with the exception of P2X6, can assemble to form homomeric or heteromeric functional channels.4-5 The different P2X receptors show distinct expression patterns. P2X1-6 has been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system. The P2X3 receptor is highly expressed on nociceptive sensory neurons in dorsal root ganglia (DRG) as a homomer or as a heteromer (P2X3/P2X2). ATP released from damaged cells activates the P2X3 receptor to initiate nociceptive signals.6,7 Involvement of ATP in the mechanism of chronic pain has been also suggested.7,8 P2X3 receptor is now becoming a possible target for the development of pain therapeutics.
References 1. Prasad, M. et al. (2001) J. Physiol. 573, 667. 2. Florenzano, F. et al. (2002) Neuroscience 115, 425. 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed.1. 4. Khakh, B.S. et al. (2001) Pharmacol. Rev. 53, 107. 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. 6. Kage, K. et al. (2002) Exp.Brain Res. 147, 511. 7. Aoki, Y. et al. (2003) Brain Res. 989, 214. 8. Burnstock, G. (2000) Br. J. Anaesth. 84, 476.
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