|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||36677 Da|
|Homology||Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical.|
|Other Names||P2Y purinoceptor 6, P2Y6, P2ry6|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CQPHDLLQKLTAKWQRQRV, corresponding to amino acid residues 311-328 of rat P2Y6 receptor (Accession number Q63371). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis and mass-spectrography.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
The nucleotide receptor P2Y6 binds UDP, and is a member of the G-protein coupled receptor superfamily.1,3 P2Y6 agonists induce an inositol phosphate/Ca2+ response that is insensitive to pertussis toxin inhibition. P2Y6 is coupled to phospholipase C via Gq, and is also coupled to inhibition of N-type Ca2+ and M-type K+ channels.1,2 P2Y6 mRNA is expressed in various rat tissues, including lung, stomach, intestine, spleen, heart, and aorta. P2Y6 was reported to mediate contractions in human cerebral arteries.4 Abgent is please to offer a highly specific antibody directed against the C-terminus domain of the rat P2Y6 receptor. Anti-P2Y6 Receptor antibody (#AG1400) can be used in western blot analysis, indirect flow cytometry, immunoprecipitation, immunocytochemistry and immunohistochemistry. It has been designed to recognize P2Y6 from rat, mouse and human samples.
References 1. Communi, D. et al. (2000) Cell Signal. 12, 351. 2. Filippov, A.K. et al. (1999) Br. J. Pharmacol. 270, 26152. 3. Chang, K. et al. (1995) J. Biol. Chem. 270, 26152. 4. Malmsjo, M. et al. (2003) B.M.C. Pharmacol. 3, 4.
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