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>   home   >   Products   >   Primary Antibodies   >   P2X4 Receptor Antibody   

P2X4 Receptor Antibody

Affinity purified polyclonal antibody

  • WB - P2X4 Receptor Antibody AG1401-025
    Western blot analysis of rat brain membranes: 1. Anti-P2X4 Receptor antibody (#AG1401), (1:200).
    2. Anti-P2X4 Receptor antibody, preincubated with the control peptide antigen.
  • IHC - P2X4 Receptor Antibody AG1401-025
    Expression of P2X4 Receptor in rat brain
    Immunohistochemical staining of rat brain red nucleus using Anti-P2X4 Receptor antibody (#AG1401). A. P2X4 Receptor (green) appears in fibers surrounding cell shapes (arrows). B. Calbindin 28K (red) appears in large neurons. C. Merge of P2X4 Receptor and Calbindin 28K suggests variable density of P2X4 Receptor expressing fibers on red nucleus neurons. DAPI is used as the counterstain (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51577
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 43501 Da
Homology Mouse - identical; human - 15/19 amino acid residues identical; chicken - 11/12 amino acid residues identical; Xenopus - 11/11 amino acid residues identical.
Additional Information
Gene ID 29659
Other Names P2X purinoceptor 4, P2X4, ATP receptor, Purinergic receptor, P2rx4
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KKYKYVEDYEQGLSGEMNQ, corresponding to amino acid residues 370-388 of rat P2X4 Receptorֲ (Accession P51577). Intracellular, C-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Application Details Western blot analysis (WB): - Mouse bone marrow-derived dendritic cells (BMDCs) (1:300) (see Sakaki, H. et al. (2013) in Product Citations). - Rat spinal cord lysate (see Lu, W.H. et al. (2013) in Product Citations). - HEK-293 cells expressing rat P2X4 receptor (see Rokic, M.B. et al. (2013) in Product Citations).   Immunoprecipitation (IP): - Rat hypothalamus lysate (see Jo, Y.H. et al. (2011) in Product Citations).   Immunohistochemistry (IH): - Primate retina (1:1000) (see Gu, B.J. et al. (2013) in Product Citations). - Rat spinal cord (see Lu, W.H. et al. (2013) in Product Citations). - Mouse brain (1:200) (see Jo, Y.H. et al. (2011) in Product Citations).   Immunocytochemistry (IC): - Rat primary microglia (see Lu, W.H. et al. (2013) in Product Citations). - Human mesangial cells (HMC) (1:200) (see Solini, A. et al. (2007) in Product Citations). Indirect flow cytometry (IFC): - NR8383 rat alveolar macrophage cell line (1:100) (see Stokes, L. and Surprenant, A. (2009) in Product Citations).  
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Citations (0)

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The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP. The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation. Seven mammalian P2X receptor subtypes (P2X1–P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N and C-termini, two membrane-spanning domains and a large extracellular loop. All P2X subunits can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only appears to function as part of a heteromeric complex.4-9 The various P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X2 receptor subunit has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes. Overexpression of P2X4 was demonstrated in microglia and in the spinal dorsal horn following peripheral nerve injury. It has been suggested that activation of P2X4 along with p38 MAPK is essential for the development of allodynia (pain from a stimulus that doesn't normally elicit pain) following nerve injury. Inhibition of P2X4 expression in spinal microglia has been suggested as a novel therapeutic approach for the treatment of allodynia.10


References 1. Prasad, M. et al. (2001) J. Physiol. 573, 667. 2. Florenzano, F. et al. (2002) Neuroscience. 115, 425. 3. Ashcroft, F.M. et al. (2000) Ion Channels and Disease Ed 1, p. 405, Academic Press, San Diego. 4. Khakh, B.S. et al. (2001) Pharmacol Rev. 53, 107. 5. Ding, Y. et al. (2000) J. Auton. Nerv. Syst. 81, 289. 6. Le, K.T. et al. (1998) J. Neurosci. 18, 7152. 7. Robertson, S.J. et al. (2001) Curr. Opin. Neurobiol. 11, 378. 8. Dunn, P.M. et al. (2001) Prog. Neurobiol. 65, 107. 9. Kim, M. et al. (2001) EMBO J. 20, 6347. 10. Inoue, K. et al. (2004) J. Pharmacol. Sci. 94, 112.

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$ 375.00
$ 475.00
$ 575.00
Cat# AG1401-025
(40 western blots)
Availability: 2-3 weeks
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