|Reactivity||Human, Mouse, Rat|
|Calculated MW||40637 Da|
|Homology||Mouse - identical; human - 13/15 amino acid residues identical.|
|Other Names||Inward rectifier potassium channel 13, Inward rectifier K(+) channel Kir71, Potassium channel, inwardly rectifying subfamily J member 13, Kcnj13|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EMNGDLEIDHDVPPE, corresponding to amino acid residues 80-94 of rat Kir7.1 (Accession O70617). Extracellular loop.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Kir7.1 (KCNJ13) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels. The family’s protein topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunits that can assembly as either homo or heteromers. Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis1. Kir7.1, an inwardly rectifying K+ channel with unusual permeation properties is localized in epithelial cells of the thyroid, small intestine, kidney tubules, choroid plexus and in retinal pigment epithelium (RPE), where it forms a major component of the apical membrane K+ conductance2. A mutation in the gene encoding the channel was found to cause snowflake vitreoretinal degeneration (SVD) which is a developmental and progressive hereditary eye disorder that affects multiple tissues within the eye3. Abgent is pleased to offer a highly specific antibody directed against an epitope located at the extracellular loop domain of the rat Kir7.1 channel. Anti-Kir7.1 (extracellular) antibody (#AG1402) can be used in Western blot, immunohistochemical and indirect flow cytometry applications, and was designed to recognize Kir7.1 from rat, mouse and human samples.
References 1. Bond, C.T. et al. (1994) Rec. Channels 2, 183. 2. Yang, D. et al. (2008) Exp. Eye Res. 86, 81. 3. Hejtmanick, J.F. et al. (2008) Am. J. Hum. Genet. 82, 174.
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