|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||134396 Da|
|Homology||Rat- identical; human, bovine, chicken, dog - 16/17 amino acid residues identical.|
|Other Names||Calcium-activated potassium channel subunit alpha-1, BK channel, BKCA alpha, Calcium-activated potassium channel, subfamily M subunit alpha-1, K(VCA)alpha, KCa11, Maxi K channel, MaxiK, Slo-alpha, Slo1, mSlo1, Slowpoke homolog, Slo homolog, mSlo, Kcnma1, Kcnma|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200 of mouse KCa1.1ֲ (Accession number Q08460). Intracellular, C-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Mouse kidney lysates (see Zhang, Y. et al. (2013) in Product Citations). - Mitochondria and mitoplast from human endothelial EA.hy926 cells (1:200) (see Bednarczyk, P. et al. (2013) in Product Citations). Immunoprecipitation (IP): - Human cardiac fibroblasts (1:750) (see Wang, Y.J. et al. (2006) in Product Citations). Immunocytochemistry (IC): - Human chorionic plate arterial smooth muscle cells (10 µg/ml) (see Brereton, M.F. et al. (2013) in Product Citations). - Mouse mesenteric smooth muscle cells (1:100) (see Suzuki, Y. et al. (2013) in Product Citations). - E9 chick CG neurons (see Jha, S. and Dryer, S.T. (2009) in Product Citations). Immunohistochemistry (IH): - Human chorionic plate arterial smooth muscle cell sections (10 µg/ml) (see Brereton, M.F. et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.4 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
KCa1.1 (BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca2+ and by membrane depolarization. KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect between intracellular Ca2+ signaling pathways and membrane excitability. Indeed, KCa1.1 channels have a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.
References 1. Hofland L.J. and Lamberts, S.W.J. (2001) Annals of Oncology 12, S31. 2. Fombonne, J. et al. (2003) Rep. Biol. Endocrinol. 1, 19. 3. Slooter, G.D. et al. (2001) Br. J. Surg. 88, 31. 4. Schulz, S. et al. (2002) Gynecologic Oncology 84, 235.
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