|Reactivity||Human, Mouse, Rat|
|Calculated MW||21909 Da|
|Homology||Mouse, human, dog, rabbit- identical.|
|Other Names||Calcium-activated potassium channel subunit beta-1, BK channel subunit beta-1, BKbeta, BKbeta1, Calcium-activated potassium channel, subfamily M subunit beta-1, Calcium-activated potassium channel subunit beta, Charybdotoxin receptor subunit beta-1, K(VCA)beta-1, Maxi K channel subunit beta-1, Slo-beta-1, Slo-beta, Slowpoke-beta, Kcnmb1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide KKLVMAQKRGETRALC, corresponding to amino acid residues 2-17 of rat slo־²1 (Accession P97678). Intracellular, N-terminal part.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat mesenteric arteries (1:400) (see Shi, L.1 et al. (2013) in Product Citations). - Rat mesenteric arteries (1:400) (see Shi, L. et al. (2013) in Product Citations). - Mouse pulmonary artery smooth muscle cells (PASMCs) (see Zheng, Y.M. et al. (2013) in Product Citations). - Mouse cerebral artery smooth muscle cells (CASMCs) (see Zheng, Y.M. et al. (2013) in Product Citations). Immunohistochemistry (IH): - Rat mesenteric arteries (1:200) (see Shi, L.1 et al. (2013) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
sloβ1 is a member of a family of regulatory β subunits that control the activity of the large conductance Ca2+-activated K+ channel KCa1.1. The family includes four members with a shared topology: two trans-membrane domains, short intracellular N- and C-termini and a large extracellular region. The four members of the family have a distinct tissue distribution with sloβ1 expressed almost exclusively in smooth muscle. Functionally, sloβ1 increases the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and voltage and it also changes its pharmacology. In the past few years there has been a lot interest regarding the role of the sloβ1 subunit in the regulation of vascular tone and hypertension. Studies in sloβ1 knockout mice have shown that loss of this subunit results in systemic hypertension. Conversely, a recent study showed that a commonly found gain-of-function sloβ1 variant has a protective effect against human diastolic hypertension.
References 1. Knaus, H-G. et al. (1994) J. Biol. Chem. 269, 17274. 2. Orio, P. et al. (2002) News Physiol. Sci. 17, 156. 3. Brenner, R. et al. (2000) Nature 407, 870. 4. Fernández- Fernández, J.M. et al. (2004) J. Clin. Invest. 113, 1032.
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