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HCN2 Antibody

Affinity purified polyclonal antibody

  • WB - HCN2 Antibody AG1406-025
    Western blot analysis of rat brain membranes:
    1. Anti-HCN2 antibody (#AG1406), (1:200).
    2. Anti-HCN2 antibody, preincubated with the control peptide antigen.
  • IHC - HCN2 Antibody AG1406-025
    Expression of HCN2 in rat cerebellum
    Immunochistochemical staining of rat cerebellum frozen sections using Anti-HCN2 antibody (#AG1406 ). A. HCN2 (red) appears in Purkinje cells (arrows). B. Staining of astrocytes with mouse anti-glial fibrillary acidic protein (GFAP, green demonstrates the restriction of HCN2 to neuronal cell bodies. C. Confocal merge of HCN2 and GFAP images demonstrates the respective localization of these proteins.
  • IHC - HCN2 Antibody AG1406-025
    Expression of HCN2 in mouse hypothalamus
    Immunohistochemical staining of mouse hypothalamus using Anti-HCN2 antibody (#AG1406). A. HCN2 (red) appears in cells of the paraventricular nucleus (PVN, arrows). B. Staining of paraventricular nerve cells with mouse anti-calcium binding protein (CBD28k, green). C. Confocal merge of HCN2 and CBD28k demonstrates some co-localization. V = Third ventricle.
  • IHC - HCN2 Antibody AG1406-025
    Colocalization of HCN4 and HCN2 in mouse thalamus Immunohistochemical staining of mouse thalamus frozen section using guinea pig Anti-HCN4 antibody (#AGP-004) and rabbit Anti-HCN2 antibody (#AG1406). A. Staining of HCN4 (green) appears in the ventral posterior thalamic nucleus (VPL). B. In the same section as in A, staining of HCN2 (red) appears in the ventral posterior thalamic nucleus (VPL) and also in the reticular thalamic nucleus (RT). The area between these thalamic nuclei (star) is white matter and neither protein is expressed in that region. C. Merged images of A and B.
  • ICC - HCN2 Antibody AG1406-025
    Expression of HCN2 in rat DRG primary culture
    Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture using Anti-HCN2 antibody (#AG1406), (1:100), (green). Cells were stained with Anti-HCN2 antibody followed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Nuclear staining of cells using the cell-permeable DNA dye Hoechst 33342 (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UL51
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 96950 Da
Homology Rat, mouse - identical.
Additional Information
Gene ID 610
Other Names Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, Brain cyclic nucleotide-gated channel 2, BCNG-2, HCN2, BCNG2
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)EEAGPAGEPRGSQAS, corresponding to amino acid residues 147-161 of human HCN2 (Accession Q9UL51). Intracellular, N-terminus.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain having crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2 The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated cannel family (HCN), which comprises of four homologous members, named HCN1-4. Each HCN subunit consist of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain to cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2 The HCN subunits can form functionally homomers and can also co-assemble into functionally heteromers.2 The channels are closely related to each other and share homology of about 60%. However, they are diverging from each other in their cytoplasmic N- and the C-terminus. The channels HCN1-4 mainly differ from each other with regard to their speed of activation and the extent by which they are modulated by cAMP.  HCN1 is the fastest channel, followed by HCN2, HCN3 and HCN4.2,3   HCN2 is the most abundant neuronal channel and is found almost ubiquitously in the brain.3   Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the HCN2 channel. The Anti-HCN2 (#AG1406) can be used for western blot, immunoprecipitation, immunohistochemical and immunocytochemical applications. It has been designed to recognize HCN2 from human, rat and mouse samples.


References 1. Much, B. et al. (2003) J.Biol.Chem. 278,  43781. 2. Notomi, T. et al. (2004) J. Comp. Neurol. 471, 241. 3. Hofmann, F. et al. (2005) Pharmacol. Rev. 57, 455.

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Cat# AG1406-025
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