|Reactivity||Human, Mouse, Rat|
|Calculated MW||96950 Da|
|Homology||Rat, mouse - identical.|
|Other Names||Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, Brain cyclic nucleotide-gated channel 2, BCNG-2, HCN2, BCNG2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)EEAGPAGEPRGSQAS, corresponding to amino acid residues 147-161 of human HCN2 (Accession Q9UL51). Intracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain having crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2 The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated cannel family (HCN), which comprises of four homologous members, named HCN1-4. Each HCN subunit consist of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain to cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2 The HCN subunits can form functionally homomers and can also co-assemble into functionally heteromers.2 The channels are closely related to each other and share homology of about 60%. However, they are diverging from each other in their cytoplasmic N- and the C-terminus. The channels HCN1-4 mainly differ from each other with regard to their speed of activation and the extent by which they are modulated by cAMP. HCN1 is the fastest channel, followed by HCN2, HCN3 and HCN4.2,3 HCN2 is the most abundant neuronal channel and is found almost ubiquitously in the brain.3 Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of the HCN2 channel. The Anti-HCN2 (#AG1406) can be used for western blot, immunoprecipitation, immunohistochemical and immunocytochemical applications. It has been designed to recognize HCN2 from human, rat and mouse samples.
References 1. Much, B. et al. (2003) J.Biol.Chem. 278, 43781. 2. Notomi, T. et al. (2004) J. Comp. Neurol. 471, 241. 3. Hofmann, F. et al. (2005) Pharmacol. Rev. 57, 455.
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