|Application ||WB, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||74699 Da|
|Homology||Rat, mouse - 14/16 amino acid residues identical.|
|Other Names||Potassium voltage-gated channel subfamily KQT member 1, IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1, KQT-like 1, Voltage-gated potassium channel subunit Kv71, KCNQ1, KCNA8, KCNA9, KVLQT1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)TYEQLTVPRRGPDEGS, corresponding to amino acid residues 661-676 of human Kv7.1 (Accession number P51787). Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Application Details||Western blot analysis (WB): - MDCK cells stably expressing human KV7.1 (1:1000) See Andersen, M.N. et al. (2013) in Product Citations). - Xenopus oocyte membrane lysates (1:500), (see Lundby, A. et al. (2013) in Product Citations). Immunoprecipitation (IP): - Canine left ventricle (8 μg) (see Ehrlich, J.R. et al. (2004) in Product Citations). Immunohistochemistry (IH): - Guinea pig detrusor (1:200) (see Anderson, U.A. et al. (2013) in Product Citations). - Mouse distal colon (1:50) (see Jepps, T.A. et al. (2009) in Product Citations). Immunocytochemistry (IC): - COS cells (see Wiener, R. et al. (2008) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.75 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||2.5 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
KV7.1 (KCNQ1) is part of a voltage-gated K+ family that includes 5 other members (KCNQ1-5). The channel has been intensively studied since it was found that together with MinK (Isk) it underlay the cardiac Iks current that controls the duration of the action potential of the human heart. Indeed mutations on either KV7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death. Although KV7.1 expression and function has been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues. There it has been implicated in physiological functions such as: regulation of acid secretion in the stomach and Cl- secretion into the colon.
References 1. Wang, Q. et al. (1996) Nat. Genet. 12, 17. 2. Grahammer, F. et al. (2001) Gastroenterology 120, 1363. 3. Mall, M. et al. (1998) Am. J. Physiol. 275, G1274.
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