|Application ||WB, IP|
|Calculated MW||65857 Da|
|Homology||Dog - identical.|
|Other Names||Potassium voltage-gated channel subfamily C member 1, NGK2, RAW2, Voltage-gated potassium channel subunit Kv31, Voltage-gated potassium channel subunit Kv4, Kcnc1|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide CKESPVIAKYMPTEAVRVT, corresponding to amino acid residues 567-585 of rat Kv3.1b (Accession P25122).ֲ ֲ Intracellular, C-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Application Details||Western blot analysis (WB): - Rat lung lysate (see Lv, Y. et al. (2013) in Product Citations). - Mouse brain lysates, also tested in KV3.1b knockout mice (see Barry, J. et al. (2013) in Product Citations). Immunocytochemistry (IC): - Mouse cultured hippocampal neurons and mouse cultured cerebellar neurons. Also tested in KV3.1b knockout mice cultured cerebellar neurons (see Barry, J. et al. (2013) in Product Citations). - Rat neural progenitor cells (NPCs) (1:200) (see Liebau, S. et al. (2006) in Product Citations). Immunohistochemistry (IH): - Mouse coronal cerebellum sections. Also tested in same sections from KV3.1b knockout mice (see Barry, J. et al. (2013) in Product Citations). - Mouse medial nucleus of the trapezoid body (MNTB) (1:500) (see Leao, K.E. et al. (2010) in Product Citations). Immunoprecipitation (IP): - Transfected COS-7 cells (see Wu, H. et al. (1998) in Product Citations).|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
KV3.1 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins KV3.2, KV3.3 and KV3.4 they constitute the Shaw type subfamily family.1 As with all KV channels, KV3.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional Kv channel is a tetramer that can either be a homomer or a heteromer of KV3 subunits. KV3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.3 KV3.1 is highly expressed in the brain but has been also detected in peripheral organs such as lung skeletal muscle and testis. KV3.1 and KV3.2 are highly enriched in neurons that fire at high frequencies, such as fast spiking interneurons of the cortex and hippocampus and neurons in the globus pallidus. Their unusually rapid activation and deactivation rates allow channels containing KV3.1 and KV3.2 subunits to repolarize action potentials quickly thus minimizing the rate of recovery of sodium channel inactivation.3 Lack of KV3.1 channel subunits is mainly responsible for constitutively increased locomotor activity and sleep loss.2
References 1. Rettig, J. et al. (1992) EMBO J. 11, 2473 2. Espinosa, F. et al. (2001) J. Neurosci. 21, 6657. 3. Rudy, B. et al. (2001) Trends Neurosci. 24, 517.
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