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Orexin Receptor 1 Antibody

Affinity purified polyclonal antibody

     
  • WB - Orexin Receptor 1 Antibody AG1413-025
    Western blot analysis of mouse brain lysate:

    1. Anti-Orexin Receptor 1 antibody (#AG1413), (1:200).
    2. Anti-Orexin Receptor 1 antibody, preincubated with the control peptide antigen.

  • WB - Orexin Receptor 1 Antibody AG1413-025
    Western blot analysis of rat brain lysate:

    1. Anti-Orexin Receptor 1 antibody (#AG1413), (1:500).
    2. Anti-Orexin Receptor 1 antibody, preincubated with the control peptide antigen.

  • WB - Orexin Receptor 1 Antibody AG1413-025
    Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lines: 1, 2. Anti-Orexin Receptor 1 antibody (#AG1413), (1:500).
    3, 4. Anti-Orexin Receptor 1 antibody, preincubated with the control peptide antigen.
  • IHC - Orexin Receptor 1 Antibody AG1413-025
    Expression of OX1R in rat brain Longitudinal frozen section of rat brainstem showing staining (red) in neuronal cell bodies. Slides were incubated overnight at 4°C with Anti-Orexin Receptor 1 antibody (#AG1413), (1:50) followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (1:500). Hoechst 33342  is used as a counterstain (blue).
  • IHC - Orexin Receptor 1 Antibody AG1413-025
    Expression of OX1R in rat colon Immunohistochemical staining of paraffin-embedded longitudinal section of rat colon showing mucosa (M), submucosa (SM), and muscularis externa (ME) using Anti-Orexin Receptor 1 antibody (#AG1413), (1:100). Note that stain (red-brown color) is highly specific for absorptive cells in the superior third of the intestinal glands. Immunolabeling was detected using DAB as the chromogen and hematoxilin as the counterstain.
  • IHC - Orexin Receptor 1 Antibody AG1413-025
    Expression of OX1R in mouse septum Immunohistochemical staining paraffin-fixed frozen sections using Anti-Orexin Receptor 1 antibody (#AG1413), (1:50). A. OX1R (green) appears in axonal processes (right-pointing triangles). B. Parvalbumin (red) appears in septal neurons. Cell nuclei (blue) are visualized with Hoechst 33342. C. Merge of OX1R and parvalbumin suggests that orexinergic innervation covers the entire septal nucleus rather than restricted to individual neurons.
  • ICC - Orexin Receptor 1 Antibody AG1413-025
    Expression of OX1R in human colon cancer cell lines Immunocytochemical staining of paraformaldehyde fixed and permeabilized human Colo-205 colon cancer cells A.  Cells were stained with Anti-Orexin Receptor 1 antibody (#AG1413), (1:200), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody. B.  Live view of the same field as in (A). C.  Nuclei were visualized with the cell permeable dye Hoechst 33342 (blue staining).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P56718
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 46800 Da
Homology Mouse - identical; human - 9/14 amino acid residues identical.
Additional Information
Gene ID 25593
Other Names Orexin receptor type 1, Ox-1-R, Ox1-R, Ox1R, Hypocretin receptor type 1, Hcrtr1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R (Accession P56718). 3rd intracellular loop.
Dilution WB~~1:200-1:2000
IHC~~1:50
ICC~~1:200
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Background

Orexin Receptor 1 (OX1R) (also known as hypocretin receptor 1) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin. OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference), while OX2R binds both ligands with similar affinities.2,3   Both OX1R and OX2R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. OX1R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels.2,3   The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research in the last few years. OX1R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2 It appears to be involved in the regulation of feeding behavior in rodents since an OX1R antagonist is able to inhibit baseline feeding.2,3    The orexin system has been shown to be involved in the regulation of sleep and wakefulness states and OX1R knockout mice show defects in the regulation of rapid eye movement (REM) sleep, among other phenotypic alterations.4 In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4   Abgent is pleased to offer a highly specific antibody directed against an epitope located in the 3rd intracellular loop of rat Orexin Receptor 1. Anti-Orexin Receptor 1 antibody (#AG1413) can be used in Western blot, immunocytochemical, and immunohistochemical applications and recognizes OX1R from rat, mouse, and human samples.

References

References 1. Sakurai, T. et al. (1998) Cell 92, 573. 2. Kukkonen, J.P. et al. (2002) Am J Physiol Cell Physiol 283, C1567. 3. Kirchgessner, A.L. (2002) Endocr Rev. 23, 1. 4. Sakurai, T. (2007) Nat. Rev. Neurosci. 8, 171.

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Cat# AG1413-025
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