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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NBCe1 (extracellular) Antibody   

NBCe1 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - NBCe1 (extracellular) Antibody AG1415-025
    Western blot analysis of rat brain lysate: 1. Anti-NBCe1 (extracellular) antibody (#AG1415), (1:200).
    2. Anti-NBCe1 (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - NBCe1 (extracellular) Antibody AG1415-025
    Western blot analysis of mouse liver membranes: 1. Anti-NBCe1 (extracellular) antibody (#AG1415), (1:400).
    2. Anti-NBCe1 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - NBCe1 (extracellular) Antibody AG1415-025
    Expression of NBCe1 in rat lateral septum Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NBCe1 (extracellular) antibody (#AG1415), (1:400). NBCe1 staining (red) is detected in blood vessel outlines (vertical arrow) in the lateral septum (LS) and along the wall of the lateral ventricle (LV) (horizontal arrow).  DAPI is used as the counterstain (blue).
  • ICC - NBCe1 (extracellular) Antibody AG1415-025
    Expression of NBCe1 in rat PC12 cells Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-NBCe1 (extracellular) antibody (#AG1415), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of the two images.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9JI66
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 121343 Da
Homology Rat - 13/14 amino acid residues identical; mouse - 12/14 amino acid residues identical.
Additional Information
Gene ID 84484
Other Names Electrogenic sodium bicarbonate cotransporter 1, Sodium bicarbonate cotransporter, NBC-like protein, Na(+)/HCO3(-) cotransporter, Solute carrier family 4 member 4, Slc4a4, Nbc, Nbc1, Nbce1, Rnbc1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)KE(S)VKFGGKLVGNN, corresponding to amino acid residues 672-685 of rat NBCe1 (Accession Q9JI66). 2nd extracellular loop, based on the 10 transmembrane topology.Not recommended for human samples. The antibody will recognize all known rat splice variants
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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SLC4 membrane transporter proteins play important roles in kidney acid–base regulation through their transport of bicarbonate (or carbonate), Na‏+, Cl-, and (possibly NH4+)1. These transporters differ in their substrate (Na+‏, Cl-) dependence, charge transport stoichiometry, cell-type and developmental expression, functional regulation, and protein–protein interactions. In mammals, SLC4 proteins are encoded by 10 different genes that share protein sequence homology and are grouped according to their functional properties.   In humans, the electrogenic sodium bicarbonate cotransporter NBCe-1 is encoded by the SLC4A4 gene2. NBCe1-A has a large N-terminal cytoplasmic region, a lipid embedded transmembrane region, and a C-terminal cytoplasmic tail3. The N-terminal cytoplasmic region is tightly folded and is predicted to form a domain structure, unlike the freely aqueous accessible C-terminal cytoplasmic tail. NBCe1-A is composed of either 10 or 14 transmembrane domains. Based on the 10 transmembrane topology, a large extracellular loop (EL) is present between TM3 and TM4 and contains two glycosylated sites. The oligomeric state of the cotransporter is dimeric and each monomeric subunit has independent transport activity4.   Mutations in NBCe1 cause autosomal recessive proximal renal tubular acidosis (pRTA). Patients with NBCe1 mutations have severe pRTA, growth and mental retardation, basal ganglia calcification, cataracts, corneal opacities (band keratopathy), glaucoma, elevated serum amylase and lipase, and defects in the enamel consistent with amelogenesis imperfect3. Mice with loss of NBCe1 have a more severe phenotype with marked volume deletion and decreased survival5.


References 1. Romero, M.F. et al. (2013) Mol. Aspects Med. 34,159. 2. Abuladze, N. et al.(2000) Gene 251, 109. 3. Zhu, Q. et al. (2010) J. Biol. Chem. 285, 13416. 4. Kao, L. et al. (2008) J. Biol. Chem. 283, 26782. 5. Igarashi, T. et al. (1999) Nat. Genet. 23, 264.

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Cat# AG1415-025
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