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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Human p75NTR(extracellular) Antibody   

Human p75NTR(extracellular) Antibody

Affinity purified polyclonal antibody

     
  • WB - Human p75NTR(extracellular) Antibody AG1417-025
    Western blot analysis of rat brain membranes:
    1. Anti-Human p75NTR (extracellular) antibody (#AG1417), (1:200).
    2. Anti-Human p75NTR (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - Human p75NTR(extracellular) Antibody AG1417-025
    Western blot analysis of human melanoma cells A875:
    1. Anti-Human p75NTR (extracellular) antibody (#AG1417), (1:200).
    2. Anti-Human p75NTR (extracellular) antibody, preincubated with the control peptide. 
  • WB - Human p75NTR(extracellular) Antibody AG1417-025
    Western blot analysis of normal rat tissue (right) and in human cancer cell lines (left): p75NTR is visualized with Anti-Human p75NTR (extracellular) antibody (#AG1417), (1:200).  Note that human cancer cell lines from hemapoietic origin show high p75NTR expression, while cell lines from prostate and colon cancer origin show lower levels.  Interestingly, p75NTR from rat (right blot and the C6 cell line) and human (left blot) samples run with a different apparent MW, probably due to species-specific differential glycosylation.
  • IP - Human p75NTR(extracellular) Antibody AG1417-025
    Immunoprecipitation of 3T3/p75NTR transfected cells:
    1. Cell lysate + protein A beads.
    2. Cell lysate + protein A beads + pre-immune rabbit serum.
    3. Cell lysate + protein A beads + Anti-Human p75NTR (extracellular) antibody (#AG1417).
    4. Cell lysate.

    Red arrows indicate the p75NTR receptor while the black arrow shows the IgG heavy chain.
    Immunoblot was performed with the Anti-Human p75NTR (extracellular) antibody.
  • IHC - Human p75NTR(extracellular) Antibody AG1417-025
    Expression of p75NTR in rat brain Immunohistochemical staining of  rat brain with Anti-Human p75NTR (extracellular) antibody (#AG1417). A. Cells in the diagonal band are stained positive for p75NTR. B. Staining of the same section with goat anti-ChAT confirms that p75NTR staining is specific to cholinergic neurons. 
  • IHC - Human p75NTR(extracellular) Antibody AG1417-025
    Expression of p75NTR in rat brain
    Immunohistochemical staining of  rat brain with Anti-Human p75NTR (extracellular) antibody (#AG1417). A. Cells in the nucleus basalis mangocellularis are stained positive for p75NTR. B. Staining of the same section with goat anti-ChAT confirms that the p75NTR staining is specific to cholinergic neurons.
  • FC - Human p75NTR(extracellular) Antibody AG1417-025
    Indirect flow cytometry analysis of A875 human melanoma cells  
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP, LCI
Primary Accession P08138
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 45183 Da
Homology Mouse - identical; rat -15/16 amino acidresidues identical.
Additional Information
Gene ID 4804
Other Names Tumor necrosis factor receptor superfamily member 16, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, p75 ICD, CD271, NGFR, TNFRSF16
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR (SwissProt accession P08138). Extracellular, stalk domain.
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl water.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Research Areas
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Background

The p75NTR receptor is a member of the TNF receptor superfamily. p75NTR, like all the superfamily members is a type I transmembrane protein with tandem cysteine-rich domains in the extracellular portion and a intracellular death domain. p75NTR binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors will determine the ligand being recognized (see below) and the biological response to its binding. As it name implies, p75NTR binds all the neurotrophins (NGF, BDNF, NT-3 and NT-4) with similar nM affinities. Co-expression of p75NTR with the Trk receptors enhances the ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF) probably as a complex with sortilin and this leads to cell death by apoptosis. Finally, a multimeric complex of p75NTR and Nogo receptor binds myelin proteins such as Nogo-66, MAG and OmgP resulting in inhibition of axonal growth. Abgent has now developed a new antibody specific for the p75NTR (#AG1417). The antibody was raised against a highly conserved epitope located in the stalk domain of the human p75NTR extracellular region. The chosen epitope makes this antibody highly suited for several applications including indirect flow cytometry, immunocytochemistry, immunohistochemistry and western blot. The antibody works equally well in mouse and rat samples.

References

References 1. Hempstead, B.L. (2002) Curr. Opin. Neurobiol. 12, 260. 2. Chao, M.V. (2003) Nat. Rev. Neurosci. 4, 299. 3. Barker, P.A. (2004) Neuron 42, 529.

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Cat# AG1417-025
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