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Nectin-1 (extracellular) Antibody

Affinity purified polyclonal antibody

  • WB - Nectin-1 (extracellular) Antibody AG1418-025
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1-2. Anti-Nectin-1 (extracellular) antibody (#AG1418), (1:200).
    3-4. Anti-Nectin-1 (extracellular) antibody, preincubated with the control peptide antigen.
  • WB - Nectin-1 (extracellular) Antibody AG1418-025
    Western blot analysis of human Jurkat T-cell leukemia (lanes 1 and 3) and human MEG-01 chronic myelogenous leukemia (lanes 2 and 4) cell lysates:
    1-2. Anti-Nectin-1 (extracellular) antibody (#AG1418), (1:200).
    3-4. Anti-Nectin-1 (extracellular) antibody, preincubated with the control peptide antigen.
  • IHC - Nectin-1 (extracellular) Antibody AG1418-025
    Expression of Nectin-1 in rat cerebellum
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-Nectin-1 (extracellular) antibody (#AG1418), (1:100). A. Nectin-1 staining (red) is apparent in Purkinje neurons and their dendritic tree (arrow). B. Cell nuclei in the same section are visualized with DAPI (blue). C. Merge of the two images.
  • ICC - Nectin-1 (extracellular) Antibody AG1418-025
    Expression of Nectin-1 in rat PC12 cells
    Immunocytochemical staining of live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-Nectin-1 (extracellular) antibody (#AG1418), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of the two images.
  • FC - Nectin-1 (extracellular) Antibody AG1418-025
    Indirect flow cytometry analysis of live intact human THP-1 monocytic leukemia cell line:
    ___ Cells + goat anti-rabbit-AlexaFluor-488 secondary antibody.
    ___ Cells + Anti-Nectin-1 (extracellular) antibody (#AG1418), (1:15) + goat anti-rabbit-AlexaFluor-488 secondary antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15223
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 57158 Da
Homology Rat - identical; mouse - 13/14 amino acid residues identical.
Additional Information
Gene ID 5818
Other Names Nectin-1, Herpes virus entry mediator C, Herpesvirus entry mediator C, HveC, Herpesvirus Ig-like receptor, HIgR, Poliovirus receptor-related protein 1, CD111, PVRL1, HVEC, PRR1
Related products for control experimentsControl peptide antigen (supplied with the antibody free of charge).
Target/Specificity Peptide (C)GKPPSVVSWETRLK, corresponding to amino acid residues 177- 190 of human nectin-1 (Accession Q15223). Extracellular, N-terminus.This antibody will recognize all known isoforms of Nectin-1, including the secreted form (also known as isoform gamma).
Dilution WB~~1:200-1:2000
Peptide Confirmation Confirmed by mass-spectrography and amino acid analysis.
Format Affinity purified antibody, lyophilized powder
Reconstitution 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.
Antibody Concentration After Reconstitution 0.8 mg/ml.
Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Storage After ReconstitutionThe reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).
Control Antigen Storage Before ReconstitutionLyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Control Antigen Reconstitution 100 µl DDW.
Control Antigen Storage After Reconstitution-20ºC.
Preadsorption Control 1 µg peptide per 1 µg antibody.
Formulation Lyophilized powder. Resuspended antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Research Areas
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Nectins, which were originally identified as virus receptors, are members of the cell-cell adhesion molecule (CAM) family. They are Ca2+-independent immunoglobulin-like CAMs. The nectin family comprises four members, nectin-1, nectin-2, nectin-3 and nectin-4, which are encoded by the PVRL1, PVRL2, PVRL3 and PVRL4 genes, respectively1.  In the central nervous system, these cell adhesion molecules aggregate in formations, termed puncta adherentia junctions, which are mechanical adhesive sites that connect pre- and postsynaptic membranes2.   All nectins contain an extracellular region with three immunoglobulin-like loops (one V type and two C2 types), a single membrane-spanning region and a cytoplasmic tail. Nectins directly bind afadin, an F-actin-binding protein, through their cytoplasmic tails1.   Nectin-1 is expressed at cell-cell junctions in human and mouse epidermis. In mice lacking the gene encoding nectin-1 (Pvrl1−/− mice), the expression of loricrin, a differentiation marker and a major component of cornified cell envelopes in the epidermis, is downregulated and newborn pups have a shiny and slightly reddish skin3.  Mutations in human PVRL1 are implicated in cleft lip or palate-ectodermal dysplasia syndromes, which includes Zlotogora–Ogur syndrome and Margarita Island ectodermal dysplasia4. Both of these are autosomal recessive disorders that are clinically characterized by unusual facial appearance, dental anomalies, hypotrichosis, palmoplantar hyperkeratosis and onychodysplasia, syndactyly, cleft lip or palate, and in some cases, mental retardation5


References 1. Rikitake, Y. et al. (2012) J. Cell Sci. 125, 3713. 2. Fantin, M. et al. (2013) PLoS One 8, e56897. 3. Wakamatsu, K. et al. (2007) J. Biol. Chem. 282, 1817. 4. Sözen, M.A. et al.  (2001) Nat. Genet. 29, 141.  5. Suzuki, K. et al.  (2000) Nat. Genet. 25, 427.

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Cat# AG1418-025
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