|Application ||WB, LCI|
|Calculated MW||90961 Da|
|Homology||Mouse - identical; human - 12/14.|
|Other Names||Neuroligin-2, Nlgn2|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)DLGPRAYDRFPGDS, corresponding to amino acid residues 657-670 of rat Neuroligin 2 (Accession Q62888). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by mass-spectrography and amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.85 mg/ml.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl DDW.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
|Formulation||Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.|
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Provided below are standard protocols that you may find useful for product applications.
Neuroligins (NLGNs) are family of postsynaptic cell adhesion molecules that play an important role in synaptic development and function1. Four genes encode for the different members of the neuroligin family in mammals: NLGN 1-4, which are differentially enriched in postsynaptic specializations of synapses2. Neuroligin 2 (NLGN2) is expressed in neurons in the brain and also in pancreatic β cells where it facilitates insulin secretion3. Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners3. NLGN2 plays a role in synapse function and synaptic signal transmission, especially via γ-aminobutyric acid receptors (GABA(A) receptors). NLGN2 modulates signaling by inhibitory synapses, and thereby plays a role in controlling the ratio of signaling by excitatory and inhibitory synapses and information processing4. Recent studied identified a loss-of-function mutation of NLGN2 in schizophrenia patients, suggesting an indispensable role of NLGN2 in regulating GABAergic functions5.
References 1. Chubykin, A.A. et al. (2007) Neuron 54, 919. 2. Kohl, C. et al. (2013) PLoS ONE 8, e5687. 3. Suckow, A.T. et al. (2008) Endocrinology 149, 6006. 4. Lee, K. et al. (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 336. 5. Sun C. et al. (2011) Hum. Mol. Genet. 20, 3042.
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