Nicotinic Acetylcholine Receptor alpha7 (extracellular) Antibody
Affinity purified polyclonal antibody
|Reactivity||Human, Mouse, Rat|
|Calculated MW||56503 Da|
|Homology||Mouse, human - identical.|
|Other Names||Neuronal acetylcholine receptor subunit alpha-7, Chrna7, Acra7|
|Related products for control experiments||Control peptide antigen (supplied with the antibody free of charge).|
|Target/Specificity||Peptide (C)KELVKNYNPLER, corresponding to amino acid residues 31-42 of rat nAChR־±7 (Accession Q05941). Extracellular, N-terminus.|
|Peptide Confirmation||Confirmed by amino acid analysis.|
|Format||Affinity purified antibody, lyophilized powder|
|Reconstitution||25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size.|
|Antibody Concentration After Reconstitution||0.8 mg/ml.|
|Buffer After Reconstitution||Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.|
|Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Storage After Reconstitution||The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min).|
|Control Antigen Storage Before Reconstitution||Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.|
|Control Antigen Reconstitution||100 µl water.|
|Control Antigen Storage After Reconstitution||-20ºC.|
|Preadsorption Control||1 µg peptide per 1 µg antibody.|
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Provided below are standard protocols that you may find useful for product applications.
Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs) and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1. These channel receptors are usually non-selective cation channels activated upon ligand binding which ultimately leads to the depolarization of postsynaptic cell membranes. To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of a subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer (homopentamers also exist) of these various subunits organized around a central pore1. However, the α7 subunit mainly forms homomeric functional structures, although functional channels have been observed with its association with α5, β2 or β3 subunits1. Interestingly, the α7 nAChR is the only subunit to be activated by two endogenous ligands: acetylcholine and choline1. All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in skeletal muscle. They are also expressed in non-neuronal cells such as bronchial epithelial cells4, as well lymphocytes5. The diversity of these receptors and their functional organization gives rise to unique properties and functions. The α4β2 receptor composition makes up a high affinity nicotinic receptor. In fact, its upregulation (mainly expressed by the increase of functional receptors at the membrane and not expression per se) is responsible for the increased appearance of binding sites following nicotine administration1,3. α7 nAChR seems to be involved in the impairment of sensory gating in schizophrenic individuals. Indeed, many polyphormisms have been detected in the gene promoter of the receptor6. There is also an association of the receptor with nociception as it, along with α2 and α10 subunits are expressed in DRGs, the nociceptive center7. Abgent is pleased to offer a highly specific antibody directed against an epitope of rat Nicotinic Acetylcholine Receptor α7 (nAChRα7). Anti-Nicotinic Acetylcholine Receptor α7 (extracellular) antibody (#AG1421) can be used in western blot, immunohistochemical, immunocytochemical and indirect flow cytometry applications. It has been designed to recognize nAChRα7 from mouse, rat and human samples.
References 1. Albuquerque, E.X. et al (2009) Physiol. Rev. 89, 73. 2. Karlin, A. et al. (1986) Ann. NY. Acad. Sci. 463, 53. 3. Kalamida, D. et al. (2007) FEBS J. 274, 3799. 4. Fu, X.W. et al. (2009) Am. J. Respir. Mol. Biol. 41, 93. 5. De Rosa, M.J. et al. (2009) Life Sci. 85, 449. 6. Potter, D. et al. (2006) Schizophr. Bull. 32, 629. 7. Haberberger, R.V. et al. (2004) Auton. Neurosci. 113, 32.
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